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Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction And Attachment Of Primary Human Dental Pulp Stem Cells In Real-Time Ex Vivo, Yeon Jee Yoo, Min Ji Kang, Hiran Perinpanayagam, Joo Cheol Park, Seung Ho Baek, Kee Yeon Kum
Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction And Attachment Of Primary Human Dental Pulp Stem Cells In Real-Time Ex Vivo, Yeon Jee Yoo, Min Ji Kang, Hiran Perinpanayagam, Joo Cheol Park, Seung Ho Baek, Kee Yeon Kum
Dentistry Publications
This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading …