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Nitric Oxide, A Survival Factor For Lens Epithelial Cells, Coral G. Chamberlain, Kylie Mansfield, Anna Cerra
Nitric Oxide, A Survival Factor For Lens Epithelial Cells, Coral G. Chamberlain, Kylie Mansfield, Anna Cerra
Faculty of Science, Medicine and Health - Papers: part A
Purpose: Nitric oxide (NO) is capable of promoting either cell death or cell survival depending on cell type and experimental conditions. In this study, the possible effects of NO on the viability of lens epithelial cells were investigated in an explant model used previously to identify cellular changes associated with posterior capsule opacification following cataract surgery.
Methods: Rat lens epithelial explants prepared from weanling rats were cultured in a serum-free medium for five days with or without the addition of the nitric oxide synthase inhibitor, L-Nω-nitro-L-arginine methyl ester (L-NAME), using the inactive enantiomer D-NAME as a control. Alternatively, …