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Two Copies Of Shiga-Like Toxin Ii-Related Genes Common In Enterohemorrhagic Escherichia Coli Strains Are Responsible For The Antigenic Heterogeneity Of The 0157:H- Strain E32511, Clare K. Schmitt, Marian L. Mckee, Alison D. O'Brien
Two Copies Of Shiga-Like Toxin Ii-Related Genes Common In Enterohemorrhagic Escherichia Coli Strains Are Responsible For The Antigenic Heterogeneity Of The 0157:H- Strain E32511, Clare K. Schmitt, Marian L. Mckee, Alison D. O'Brien
Uniformed Services University of the Health Sciences
Thirty-two clinical isolates of Shiga-like toxin (SLT)-producing Escherichia coli associated with single cases or outbreaks of bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, or edema disease of swine were examined for multiple copies of genes belonging to the slt-I or slt-II toxin families. Five of 19 strains that were known to produce SLT-II or to hybridize to slt-II-specific probes by colony blot were found by Southern hybridization to contain two copies of toxin genes related to slt-II. The genes for two toxins closely related to slt-II were cloned from one of the isolates, Escherichia …
Evaluation Of The Role Of Shiga And Shiga-Like Toxins In Mediating Direct Damage To Human Vascular Endothelial Cells, Vernon L. Tesh, James E. Samuel, Liyanage P. Perera, John B. Sharefkin, Alison D. O'Brien
Evaluation Of The Role Of Shiga And Shiga-Like Toxins In Mediating Direct Damage To Human Vascular Endothelial Cells, Vernon L. Tesh, James E. Samuel, Liyanage P. Perera, John B. Sharefkin, Alison D. O'Brien
Uniformed Services University of the Health Sciences
Infection with Shinga toxin- and Shinga-like toxin -producing strains of Shigella dysenteriae and Escherichia coli, respectively, can progress to the hemolytic-uremic syndrome. It has been hypothesized that circulating Shinga toxin, Shinga-like toxins, and endotoxins may contribute to the disease by directly damaging glomerular endothelial cells. The effects of these toxins on HeLa, Vero, and human vascular endothelial cells (EC) were examined. Confluent EC were sensitive to Shinga toxin but were at least 106-fold less sensitive to the toxins than were Vero cells. Shinga toxin was the predominant cytotoxic factor. Lipopolysaccharides were not cytotoxic and did not augment …