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Role Of Mirna-Mrna Interaction In Neural Stem Cell Differentiation Of Induced Pluripotent Stem Cells, Satish Kumar, Joanne E. Curran, Erica De Leon, Ana C. Leandro, Tom E. Howard, Donna M. Lehman, Sarah Williams-Blangero, David C. Glahn, John Blangero
Role Of Mirna-Mrna Interaction In Neural Stem Cell Differentiation Of Induced Pluripotent Stem Cells, Satish Kumar, Joanne E. Curran, Erica De Leon, Ana C. Leandro, Tom E. Howard, Donna M. Lehman, Sarah Williams-Blangero, David C. Glahn, John Blangero
School of Medicine Publications and Presentations
miRNA regulates the expression of protein coding genes and plays a regulatory role in human development and disease. The human iPSCs and their differentiated progenies provide a unique opportunity to identify these miRNA-mediated regulatory mechanisms. To identify miRNA–mRNA regulatory interactions in human nervous system development, well characterized NSCs were differentiated from six validated iPSC lines and analyzed for differentially expressed (DE) miRNome and transcriptome by RNA sequencing. Following the criteria, moderated t statistics, FDR-corrected p-value ≤ 0.05 and fold change—absolute (FC-abs) ≥2.0, 51 miRNAs and 4033 mRNAs were found to be significantly DE between iPSCs and NSCs. The miRNA target …
Ultra-High-Field Imaging Reveals Increased Whole Brain Connectivity Underpins Cognitive Strategies That Attenuate Pain, Enrico Schulz, Anne Stankewitz, Anderson M. Winkler, Stephanie Irving, Viktor Witkovsky, Irene Tracey
Ultra-High-Field Imaging Reveals Increased Whole Brain Connectivity Underpins Cognitive Strategies That Attenuate Pain, Enrico Schulz, Anne Stankewitz, Anderson M. Winkler, Stephanie Irving, Viktor Witkovsky, Irene Tracey
School of Medicine Publications and Presentations
We investigated how the attenuation of pain with cognitive interventions affects brain connectivity using neuroimaging and a whole brain novel analysis approach. While receiving tonic cold pain, 20 healthy participants performed three different pain attenuation strategies during simultaneous collection of functional imaging data at seven tesla. Participants were asked to rate their pain after each trial. We related the trial-by-trial variability of the attenuation performance to the trial-by-trial functional connectivity strength change of brain data. Across all conditions, we found that a higher performance of pain attenuation was predominantly associated with higher functional connectivity. Of note, we observed an association …
Highly Efficient Induced Pluripotent Stem Cell Reprogramming Of Cryopreserved Lymphoblastoid Cell Lines, Satish Kumar, Joanne E. Curran, Erika C. Espinoza, David C. Glahn, John Blangero
Highly Efficient Induced Pluripotent Stem Cell Reprogramming Of Cryopreserved Lymphoblastoid Cell Lines, Satish Kumar, Joanne E. Curran, Erika C. Espinoza, David C. Glahn, John Blangero
School of Medicine Publications and Presentations
Tissue culture based in-vitro experimental modeling of human inherited disorders provides insight into the cellular and molecular mechanisms involved and the underlying genetic component influencing the disease phenotype. The breakthrough development of induced pluripotent stem cell (iPSC) technology represents a quantum leap in experimental modeling of human diseases, providing investigators with a self-renewing and thus unlimited source of pluripotent cells for targeted differentiation into functionally relevant disease specific tissue/cell types. The existing rich bio-resource of Epstein-Barr virus (EBV) immortalized lymphoblastoid cell line (LCL) repositories generated from a wide array of patients in genetic and epidemiological studies worldwide, many of them …