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Intracellular Trafficking And Distribution Of Cd And Inp Quantum Dots In Hela And Ml-1 Thyroid Cancer Cells, Min Zhang
MSU Graduate Theses
The study of the interaction of engineered nanoparticles, including quantum dots (QDs), with cellular constituents and the kinetics of their localization and transport, has provided new insights into their biological consequences in cancers and for the development of effective cancer therapies. The present study aims to elucidate the toxicity and intracellular transport kinetics of CdSe/ZnS and InP/ZnS QDs in late-stage ML-1 thyroid cancer using well-tested HeLa cells as a control. The XTT viability assay showed that ML-1 cells, and non-cancerous mouse fibroblast cells, exhibit no viability defect in response to these QDs, whereas HeLa cell viability decreases. These results suggest …
Identifying And Comparing Transcriptome Alterations In Saccharomyces Cerevisiae Exposed To A Variety Of Quantum Dots, Cullen Horstmann
Identifying And Comparing Transcriptome Alterations In Saccharomyces Cerevisiae Exposed To A Variety Of Quantum Dots, Cullen Horstmann
MSU Graduate Theses
The primary focus of my research was to obtain global gene expression profiles of baker’s yeast exposed to sub-lethal doses of nanoparticles, such as silver nanoparticles (AgNPs), yellow- emitting CdSe/ZnS quantum dots (QDs), green-emitting CdSe/ZnS QDs, and InP/ZnS QDs, to reveal novel insights on their unique mechanisms of toxicity. Despite their diverse applications, their long-lasting effects on the environment and human health are not well understood. To assess their toxicity, I administered experiments that exposed Saccharomyces cerevisiae to a variety of nanoparticles and measured cell viability, ROS levels, and changes in gene expression. Most notably, I used RNA-sequencing (RNA-seq) to …