Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Entire DC Network
The Role Of Actin Associated Sptbn1 Host Factor In Hiv-1 Infection In Microglial Cells, Hannah Matheney
The Role Of Actin Associated Sptbn1 Host Factor In Hiv-1 Infection In Microglial Cells, Hannah Matheney
MSU Graduate Theses
Human immunodeficiency virus (HIV) is a chronic illness affecting more than 32.7 million individuals worldwide. The virus infects immune cells, weakening the immune system overtime eventually leading to acquired immunodeficiency syndrome (AIDS) if left untreated. Infection starts with a fusion step, followed by uncoating of the HIV capsid once in the cytoplasm of the cell. HIV uses host cell proteins to complete the infection process, like the actin associated factor, SPTBN1. The Hulme lab has previously shown that SPTBN1 knockdown by siRNA in microglial cells decreased HIV infection and delayed uncoating of HIV. Because fusion is prior to uncoating, it …
The Role Of The Host Factor Sptbn1 In Hiv-1 Infection Of Microglial Cells, Marc Gordon Havlicek
The Role Of The Host Factor Sptbn1 In Hiv-1 Infection Of Microglial Cells, Marc Gordon Havlicek
MSU Graduate Theses
HIV-1 is the etiological agent that cause AIDS. Since the 1980’s when HIV-1 was discovered, much has been discovered, however a cure for HIV-1 has eluded researchers. Effective therapeutics do exist but can have adverse side effects including HAND/HAD which leads to neurodegeneration in patients regardless of viral suppression. One area of research that holds the possibility of discovering new viral targets and therapeutics is host factor interaction within the replication process. One host factor that can cause a decrease in HIV-1 infectivity is SPTBN1. SPTBN1 is a cytoskeletal protein that was shown to bind to the capsid and nucleocapsid …
Role Of Cyclophilin A And Trim5Α In Hiv-1 Infection And Capsid Uncoating, Emma Lk Wise
Role Of Cyclophilin A And Trim5Α In Hiv-1 Infection And Capsid Uncoating, Emma Lk Wise
MSU Graduate Theses
Microglial cells are immune cells that protect the central nervous system and are subject to HIV- 1 infection. HIV-1 infection of these cells can lead to HIV Associated Neurocognitive Disorders (HAND). Since HIV-1 only codes for 15 different proteins, the virus also uses cellular factors, like Cyclophilin A (CypA), to assist in viral replication. The cell also has antiretroviral restriction factors, like tripartite motif-containing protein 5 (TRIM5α), to protect against infection. The goal of this project is to investigate the role of CypA in the early steps of HIV-1 replication and TRIM5α restriction in microglial cells. I hypothesize that CypA …