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Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …

Functional And Physiological Role Of Extra-Hypothalamic Corticotropin Releasing Hormone Neurons In The Nucleus Of The Hippocampal Commissure In Regulation Of Stress Response, Hakeem Kadhim

Graduate Theses and Dissertations

Corticotropin releasing hormone (CRH) neurons located within the paraventricular nucleus (PVN) are known to be involved in regulation of stress responses. Recently, CRH neurons were identified above the PVN within the nucleus of the hippocampal commissure (NHpC) that located in the septum. We hypothesized that CRH neurons in the NHpC play a critical role in the stress response due to their rapid activation and could be a part of the traditional hypothalamo-pituitary-adrenal (HPA) axis. The dissertation addresses the role of 1) CRH expressing neurons in the NHpC compared with those within the PVN utilizing two different stressors, food deprivation (FD) …

Understanding Radiation Resistance In Head And Neck Tumor Xenografts Using Diffuse Reflectance And Raman Spectroscopy, Sina Dadgar

Graduate Theses and Dissertations

Each year, 800,000 new patients are diagnosed with head and neck squamous cell carcinoma (HNSCC), a majority of whom are treated with a combination of daily fractions of radiation and weekly chemotherapy sessions for up to seven weeks. Current methods to evaluate treatment response of individual patients are limited to anatomical measurements of tumor burden using CT scan or MRI 4-8 weeks after completion of treatment. However, earlier knowledge of radiation-response prior to or at early days after commencement of therapy can aid oncologist with escalating and de-escalating treatment plans for exceptionally non-responding and responding patients. Such a knowledge can …

Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández

Graduate Theses and Dissertations

To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …

Fate And Dissemination Of Salmonella Reading In Market-Age Turkeys At Processing Using Oral Gavage Challenge Model, Aaron Ashcraft

Graduate Theses and Dissertations

This study aimed to evaluate the fate and dissemination of Salmonella Reading (SR) in market-age turkeys using an oral gavage challenge model. One hundred twenty-eight-week-old commercial turkey hens were moved from commercial production to research facilities. Upon arrival, a combination of enrofloxacin, 10 mg/kg, and florfenicol, 20 mg/kg, were orally administered sequentially before comingled placement on fresh pine shavings. Turkeys were challenged with 108 cfu SR by oral gavage on days 4 and 7 post-placement. Subsets were subjected to simulated commercial processing on days 14 (n=40), 21 (n=40) and 28 (n=32) post-placement (corresponding to 10, 11, and 12 weeks of …

Effect Of The Prospect Of Transport On Captive Tiger Behavior And Fecal Cortisol In Naïve And Experienced Tigers, Callan Lichtenwalter

Graduate Theses and Dissertations

With close to 5,000 captive tigers in the United States, it is vital to ensure that these tigers are receiving adequate welfare whether they are in an accredited facility, or privately owned. The goal of this study was to assess whether captive tigers at a rescue facility with experience being transported outside of the facility would respond differently to the presentation of their transport vehicle than their naïve counterparts who had only been transported within the facility. The behavior of 5 naïve and 7 experienced tigers (n = 12) located at Turpentine Creek Wildlife Refuge in Eureka Springs, Arkansas was …

Hpv Vaccination In Females: The Role Of Primary Health Care And Primary Health Care Providers, Brooke Bouza

Graduate Theses and Dissertations

Two reasons for subpar coverage of the Human papillomavirus (HPV) vaccine are missed clinical opportunities and nonacceptance of the vaccine upon recommendation from a medical provider. The purpose of study one was to examine factors associated with access and adherence to physician’s recommendations in association with HPV vaccine uptake. Data were obtained from National Health Interview Survey (2017). Variables theoretically related to clinical opportunities and adherence to physician’s recommendations were examined in association with HPV vaccine uptake. Univariate logistic regressions were run to determine the associations between the aforementioned variables and uptake of the HPV vaccine. Multinomial logistic regressions were …

The Adult Public’S Perception Of The Utility Of Play Therapy, Margaret L. Hindman

Graduate Theses and Dissertations

Children communicate through symbolism and play as toys are children's words and play is their language (Landreth, 2012; Oaklander, 1988; Piaget, 1951; Ray, 2016). Play therapists facilitate developmentally appropriate support for children (Landreth, 2012). Often parents are unaware of the importance of play in children’s counseling (Brumfield & Christensen, 2011; Landreth, Bratton, Kellam, & Blackard, 2006).

Since adults often make decisions for children, it is important to know adults’ perceptions of play therapy utility. There is little research on parents’ knowledge of mental health services especially research specific to play therapy (Gallo, Comer, & Barlow, 2013; O’Connor & Langer, 2018). …

Rejection Sensitivity And Social Support As Predictors Of Peer Victimization Among Youth With Psychiatric Illness, Katherine C. Hyde

Graduate Theses and Dissertations

In this study, I examined whether rejection sensitivity and perceptions of social support predicted concurrent peer victimization in a sample of adolescents with psychiatric illness. Participants included 43 adolescents, aged 12-18 with diverse psychiatric diagnoses, who were recruited from a summer residential treatment program. Participants completed measures of peer victimization, perceptions of social support, and rejection sensitivity. Participants also completed the global victimization item in the Revised Olweus Bully/Victim Questionnaire, which allowed for comparison of rates of peer victimization across studies (Solberg & Olweus, 2003). Results replicate and extend previous research that indicates adolescents with psychiatric illness experience high rates …

Within-Person Variability Of Internal And External Sexual Consent, Malachi Willis

Graduate Theses and Dissertations

Background: Sexual consent is often conceptualized as an internal willingness to engage in sexual activity, which can be communicated externally to a sexual partner. Preliminary evidence indicates that people’s sexual consent varies from day to day. Study designs that assess sexual consent at multiple time points (e.g., experience sampling methodology [ESM]) are needed to better understand the within-person variability of sexual consent; however, extant validated measures of sexual consent are not appropriate for ESM studies, which require shorter assessments due to the increased burden this methodology has on participants. As such, the goal of this dissertation was to develop valid …

Food Safety Risk In An Indoor Microgreen Cultivation System, Gina Marie Misra

Graduate Theses and Dissertations

Microgreens are immature sprouts of edible plants, sharing some similarities with sprouted seeds and petite leafy greens. Since they are most often grown in containers in buildings or greenhouses, they present a new area for food safety research at the intersection of the built environment and produce farming. Contamination by human pathogens has been extensively studied in other types of produce typically eaten raw, including sprouted seeds, which have been implicated in numerous outbreaks of salmonellosis over the last several decades. There is a paucity of knowledge about the microgreen sector of the fresh-cut industry; thus, it was determined that …

An Experimental Test Of The Effects Of Sleep Deprivation On Approach Behavior, Rebecca L. Campbell

Graduate Theses and Dissertations

Emotion regulation and sleep have been identified as mechanisms that may be involved in the development and maintenance of many mental health disorders. However, there has been little research into the relation between sleep and emotion regulation. To address this gap in knowledge, a novel study was conducted. We hypothesized that sleep deprived individuals would demonstrate less approach behavior toward a negatively valenced stimulus, as well as increased self-reported avoidance, compared to a control group. To test this, a randomized controlled experiment using a behavioral measure of approach and a self-report measure of avoidance was conducted. Fifty-two healthy individuals ages …

Cloning, Protein Expression, And Characterization Of Interleukin 1 Alpha, Musaab Habeeb Ali Al Ameer

Graduate Theses and Dissertations

Recombinant DNA technology and the ability to produce recombinant proteins have significantly changed the world of pharmaceutical market. Recombinant DNA technology using E. coli cells has successfully produced many therapeutic proteins. In this study, the designed tag Ark-RUBY-tag facilitates rapid, and reproducible purification of recombinant proteins expressed as inclusion bodies in E. coli cells. Purification of Ark-RUBY-fused recombinant protein(s) can be obtained by using imidazole fractions. Target protein can be easily removed from the Ark-RUBY-tag by enzymatic cleavage. Ark-RUBY-fused recombinant proteins can be quantitatively detected using polyclonal antibodies. Ark-RUBY-tag can be used to purify small peptides. By using Ark-RUBY-tag, purified …

Perceptions Of Help-Seeking Likelihood For Depression: Examining The Relative Predictive Value Of Ethnicity And Barriers To Care, Linda Esperanza Guzman

Graduate Theses and Dissertations

Latinx and non-Latinx White adults 18 years of age and older experience depression at proportional rates. However, Latinxs seek specialized care for depression at lower rates than non-Latinx Whites, suggesting these groups experience barriers in accessing care differently. This study sought to test the theoretical steps of help-seeking as informed by the Gaining Access and Treatment Equity model (GATE model; Bridges, 2018). According to the GATE model, successful help-seeking means navigating a series of barriers: sequentially, these are perceived need, attitudinal barriers, and structural barriers. Participants (N = 987) were either Latinx (n = 437) or non-Latinx White (n = …

Practitioner Use Of And Attitudes Toward Video-Conferencing For The Delivery Of Evidence-Based Telemental Health Interventions, Kathryn Parisi

Graduate Theses and Dissertations

Compared to telehealth delivery of interventions for physical health issues, the implementation of evidence-based psychosocial interventions using technology has less support. Video-conference delivery (VCD) has the potential to increase accessibility to effective treatments, although its use remains limited and understudied. This study employed a mixed methods approach in surveying mental health practitioners about their attitudes regarding the use of video-conference methods to deliver evidence-based interventions. One hundred and eleven practitioners were sampled from several national and regional practice organizations and administered quantitative surveys about their use of and attitudes towards VCD of evidence-based interventions. The relationship between clinician-level technology access, …

Associations Of Sex, Gender, And Gender Role Beliefs With Mental Health Attitudes, Ariel Erazo

Graduate Theses and Dissertations

Mental health stigma is a strong deterrent for individual with mental illness utilizing mental healthcare. Individuals living with a mental illness experience shame and marginalization due to stigma. Stigma is perpetuated through stereotypes created and used by people in the majority. Studies have been done to assess individual’s attitudes toward mental healthcare seeking in regards to gender, age, race, and profession. This study aimed to look at traditional gender role beliefs, sex, and gender expression as predictors of individual’s mental health attitudes and individual’s views of mental health norms. Using a cross-sectional survey, 392 participants completed scales that included the …

An Interdisciplinary Study To Understand Treatment Seeking Behavior Among Female Survivors Of Anorexia Nervosa, Meaghan Victoria Tipton

Graduate Theses and Dissertations

This study was done to examine what psychosocial factors influence treatment seeking behavior among female survivors of eating disorders. This study uses a mixed methods survey consisting of open-ended item questions regarding treatment experience and social environment, and close-ended items to measure constructs from the Health Belief Model. Fourteen participants were recruited for this study to retrospectively detail their experiences of treatment during their eating disorder. All fourteen participants identified as Caucasian, thirteen identified as female, one identified with other gender, each with a history of medically diagnosed anorexia nervosa. The mean of age of current participants was 29.46, and …

Peptoid And Antibody-Based Gfp Sensors, Solomon Isu

Graduate Theses and Dissertations

In this work, we have made and characterized a pair of immunobiosensors for detecting the green fluorescent protein (GFP) in an aqueous matrix. An anti-GFP antibody-based biosensor was assembled to detect GFP, while a novel peptoid (N-substituted oligomers of glycine designated as IOS-1) biosensor was also assembled for GFP detection. A quartz crystal microbalance (QCM) gold sensor was used as the supporting substrate for self-assembly of the immunobiosensors. Gravimetric measurements of the QCM gold sensor during immunobiosensor construction and operation were available in real-time using a QCM instrument. X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and Fluorescence microscopy were used …