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A Comparative Cytotoxic Evaluation Of Acrylamide And Diacetone Acrylamide To Investigate Their Suitability For Holographic Photopolymer Formulations, Dervil Cody, Alan Casey, Izabela Naydenova, Emilia Mihaylova
A Comparative Cytotoxic Evaluation Of Acrylamide And Diacetone Acrylamide To Investigate Their Suitability For Holographic Photopolymer Formulations, Dervil Cody, Alan Casey, Izabela Naydenova, Emilia Mihaylova
Articles
The comparative cytotoxic evaluation of two monomers Diacetone Acrylamide (DA) and Acrylamide (AA), used in holographic photopolymer formulations, is reported. Two normal cell lines were used: BEAS-2B and HaCaT. Cellular viability was assessed using the MTT assay for three different exposure times. A difference of two orders of magnitude is observed in the Lethal Dose (LD50) concentrations of the two monomers. Diacetone Acrylamide exhibits a significantly lower toxicity profile in comparison to Acrylamide at all exposure times. This result justifies the replacement of Acrylamide with Diacetone Acrylamide in the photopolymer formulation, with the view to reducing occupational hazard risks for …
Generation Of Intracellular Reactive Oxygen Species And Genotixity Effect To Exposure Of Nanosized Polyamidoamine (Pamam) Dendrimers In Plhc-1 Cells In Vitro, Pratap Naha, Hugh Byrne
Generation Of Intracellular Reactive Oxygen Species And Genotixity Effect To Exposure Of Nanosized Polyamidoamine (Pamam) Dendrimers In Plhc-1 Cells In Vitro, Pratap Naha, Hugh Byrne
Articles
Polyamidoamine (PAMAM) dendrimers elicit systematically variable cyto- and eco-toxic responses, promising the basis for structure activity relationships governing nanotoxicological responses. Increased production of intracellular reactive oxygen species (ROS), genotoxicity and apoptosis due to in-vitro exposure of hepatocellular carcinoma cells to dendrimer generations G-4, G-5 and G-6 is demonstrated. A generation dependent increase in ROS and genotoxicity was observed, consistent with our previous studies. The responses correlate well with the number of surface amino groups per generation. Although ROS production initially increases approximately linearly, it saturates at higher doses. Notably, normalised to molar dose of surface amino groups, the dose-dependent ROS …