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Growth, Detection, Quantification, And Inactivation Of Sars-Cov-2, James Brett Case, Adam L. Bailey, Arthur S. Kim, Rita E. Chen, Michael S. Diamond
Growth, Detection, Quantification, And Inactivation Of Sars-Cov-2, James Brett Case, Adam L. Bailey, Arthur S. Kim, Rita E. Chen, Michael S. Diamond
2020-Current year OA Pubs
Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 is the agent responsible for the coronavirus disease 2019 (COVID-19) global pandemic. SARS-CoV-2 is closely related to SARS-CoV, which caused the 2003 SARS outbreak. Although numerous reagents were developed to study SARS-CoV infections, few have been applicable to evaluating SARS-CoV-2 infection and immunity. Current limitations in studying SARS-CoV-2 include few validated assays with fully replication-competent wild-type virus. We have developed protocols to propagate, quantify, and work with infectious SARS-CoV-2. Here, we describe: (1) virus stock generation, (2) RT-qPCR quantification of SARS-CoV-2 RNA; (3) detection of SARS-CoV-2 antigen by flow cytometry, (4) quantification of infectious …