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A Novel Impedance Biosensor For Measurement Of Trans-Epithelial Resistance In Cells Cultured On Nanofiber Scaffolds, Robert A. Schramm, Matthew H. Koslow, Deirdre A. Nelson, Melinda Larsen, James Castracane

Biological Sciences Faculty Scholarship

Nanofibrous scaffolds provide high surface area for cell attachment, and resemble the structure of the collagen fibers which naturally occur in the basement membrane and extracellular matrix. A label free and non-destructive method of assessing the interaction of cell tissue and scaffolds aids in the ability to discern the effective quality and magnitude of any scaffold modifications. Impedance cell spectroscopy is a biosensing method that employs a functional approach to assessing the cell monolayer. The electrical impedance barrier function of a cell monolayer represents the level of restriction to diffusion of charged species between all adjacent cells across an entire …