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Investigations Into The Use Of The M-Protein For The Production Of A Vaccine Against Streptococcus Equi Subspecies Equi Using Chicken Igy, Rachel Lantz
Senior Honors Theses
Strangles is a destructive disease in horses caused by an infection of the bacteria Streptococcus equi subspecies equi. Current vaccines designed to protect horse populations for this infection are only 50% effective at preventing the disease. The aim of this study was to develop a vaccine against Streptococcus equi by targeting the antiphagocytic virulence factor of the bacteria, the M-Protein (SeM18). SeM18 was expressed using the ampicillin resistant pET vector system and purified by metal affinity column using Cu(II). Purified protein was used to vaccinate chickens, and the anti-SeM18 IgY present in the yolk of the eggs produced by …