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Synthesis And Identification Of An Investigational Essential Precursor Compound For The Purpose Of The Development Of A Vaccine Treatment Against The Peanut Allergy, Thomas F. Anguella
Synthesis And Identification Of An Investigational Essential Precursor Compound For The Purpose Of The Development Of A Vaccine Treatment Against The Peanut Allergy, Thomas F. Anguella
Theses and Dissertations
Presented in this master's thesis are several studies carried out to determine the viability of several allergoid candidates utilizing the major peanut allergen Ara h 2. The Ara h 2 allergen protein appears naturally as a doublet when observed by gel electrophoresis, SDS-PAGE. Optimization of allergen purification methods successfully led to Ara h 2 purity, and the ability to standardize procedures to yield consistently pure samples. The purified allergen was chemically crosslinked with diketone derivatives selected for their abilities to react with specific amino acids accessible on the Ara h 2 protein. Chemically modified allergen samples were also evaluated using …