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- BYL719 (1)
- Basic fibroblast growth factor (1)
- Beta cell (1)
- Cancer cells (1)
- Combination therapy (1)
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- Development (1)
- Diabetes (1)
- Differentiation (1)
- Electrospun scaffold (1)
- GLUT2 (1)
- Gingival fibroblasts (1)
- Guided tissue regeneration (1)
- Iron (1)
- MTORC1 (1)
- MagA (1)
- Magnetic resonance imaging (1)
- Mechanisms of resistance (1)
- Oral epithelium (1)
- PI3K/AKT signalling (1)
- Pancreas (1)
- Polycaprolactone (1)
- Progenitor cell (1)
- Relaxation rates (1)
- SOX2 (1)
Articles 1 - 4 of 4
Full-Text Articles in Entire DC Network
Pancreatic Beta Cell Plasticity And Involvement Of Insulin-Expressing Progenitor Cells, Christine A. Beamish
Pancreatic Beta Cell Plasticity And Involvement Of Insulin-Expressing Progenitor Cells, Christine A. Beamish
Electronic Thesis and Dissertation Repository
Islet transplants have been successfully used as treatment for diabetes, but are limited by shortages of cadaveric insulin-producing β-cells. An alternate source may be the dedifferentiation, expansion, and subsequent redifferentiation of pancreatic islets or β-cells using in vitro techniques prior to transplant. Based on protocols which described the dedifferentiation of human islets to ductal-like cells, we hypothesized that neonatal mouse islets could be similarly dedifferentiated in vitro. Dedifferentiation techniques produced significant duct-like cells, but redifferentiation to insulin-expressing cells was limited. RIPCre;Z/AP+/+ mice were consequently utilized to lineage trace β-cell fate during culture by a human placental alkaline phosphatase (HPAP) reporter. …
Influence Of Polycaprolactone And Basic Fibroblast Growth Factor On Gingival Fibroblasts, Sarah Michelsons
Influence Of Polycaprolactone And Basic Fibroblast Growth Factor On Gingival Fibroblasts, Sarah Michelsons
Electronic Thesis and Dissertation Repository
Guided tissue regeneration (GTR) to regenerate periodontal tissue involves placement of a cell-occlusive barrier membrane functionally excluding the gingiva and associated oral epithelium from the periodontal defect. Gingival connective tissue (CT) contains a rich vascular plexus and is a source of progenitor cells which could contribute to periodontal regeneration. We propose the use of a novel biodegradable and bioactive electrospun fibrous polycaprolactone (PCL) scaffold loaded with microspheres releasing basic fibroblast growth factor (bFGF) to promote gingival CT growth while maintaining a barrier to the oral epithelium. Scaffolds supported human gingival fibroblast proliferation and mesenchymal cell infiltration in a bFGF dose …
Genomic Predictors Of Drug Response To The Alpha-Specific Phosphoinositol 3-Kinase (Pi3ka-Alpha) Inhibitor Byl719 In Head And Neck Cancers, Giananthony T. Rizzo
Genomic Predictors Of Drug Response To The Alpha-Specific Phosphoinositol 3-Kinase (Pi3ka-Alpha) Inhibitor Byl719 In Head And Neck Cancers, Giananthony T. Rizzo
Electronic Thesis and Dissertation Repository
PIK3CA is the only frequently mutated, druggable oncogene in head and neck squamous cell cancer (HNSCC), with PIK3CA point mutations and gene amplification rates of 17.5% and 40% respectively, with higher rates in HPV-positive disease. The objective of this research was to determine the effects of BYL719, an α-specific PI3K inhibitor in HNSCC cell lines.
All cell lines with PIK3CA hotspot point mutations or gene amplifications will be sensitive to BYL719.
Twenty-eight HNSCC cell lines were subjected to increasing concentrations of BYL719 and cell viability was measured over time. Cell lines were screened for activating PIK3CA hotspot mutations and amplifications …
Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Electronic Thesis and Dissertation Repository
Using magnetic resonance imaging, relaxation rate measurements were
performed in cancer cells overexpressing a magnetotactic bacterial gene, MagA.
Measurements of magnetic resonance relaxation rates in this expression
system is important for optimizing cell detection and specificity, for developing
quantification methods, and for refinement of gene-based iron contrast using
magnetosome associated genes. We measured the total transverse
relaxation rate (R2*), its irreversible and reversible components (R2 and R2′,
respectively) and the longitudinal relaxation rate (R1) in MDA-MB-435 tumor cells.
Clonal lines overexpressing MagA were cultured in the presence and absence of
iron supplementation, and mounted in a …