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Modeling And Experimentation Of Drying Of Adipose Derived Adult Stem Cells, Surbhi Mittal
Modeling And Experimentation Of Drying Of Adipose Derived Adult Stem Cells, Surbhi Mittal
LSU Master's Theses
Development of protocols for storing desiccated cells at ambient temperatures offers tremendous economic and practical advantages over traditional storage procedures such as cryopreservation and freeze-drying. An integrated frame work of experimentation and modeling is adopted in the present work to develop procedures for storage of adult stem cells at ambient temperature. As a first step, we have measured the post-rehydration membrane integrity of two passages, Passage-0 (P0) and Passage-1 (P1), of human adipose-derived stem cells (hASCs). hASCs were dried using a convective stage at three different drying rates (slow, moderate and rapid) in D-PBS with trehalose (50 mM) and glycerol …