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Design And Testing Of A Biological Microelectromechanical System For The Injection Of Thousands Of Cells Simultaneously, Gregory Herlin Teichert
Design And Testing Of A Biological Microelectromechanical System For The Injection Of Thousands Of Cells Simultaneously, Gregory Herlin Teichert
Theses and Dissertations
The ability to inject DNA and other foreign particles into cells, both germ cells (e.g. to produce transgenic animals) and somatic cells (e.g. for gene therapy), is a powerful tool in genetic research. Nanoinjection is a method of DNA delivery that combines mechanical and electrical methods. It has proven to have higher cell viability than traditional microinjection, resulting in higher integration per injected embryo. The nanoinjection process can be performed on thousands of cells simultaneously using an array of microneedles that is inserted into a monolayer of cells. This thesis describes the needle array design requirements and the fabrication process …
Mathematical Model And Experimental Exploration Of The Nanoinjector Lance Array, Nathan C. Toone
Mathematical Model And Experimental Exploration Of The Nanoinjector Lance Array, Nathan C. Toone
Theses and Dissertations
The Nanoinjector Lance Array has been developed to inject foreign material into thousands of cells at once using electrophoresis to attract and repel particles to and from the electrically-charged lances. A mathematical computer model simulating the motion of attracted or repelled proteins informs the design of the nanoinjection lance array system. The model is validated by accurately predicting protein velocity in electrophoresis experiments. A complete analysis of parameters is conducted via simulations and specific research questions regarding the counter electrode of the nanoinjector lance array system are explored using the model. A novel technique for fabricating lance arrays from collapsed …