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Laser-Induced Forward Transfer (Lift) Based Bioprinting Of The Collagen I With Retina Photoreceptor Cells, Md Shakil Arman

Theses and Dissertations

This thesis focuses on the 3D bioprinting of retina cone cells using the laser-induced forward transfer (LIFT) technique. Bioprinting has a great potential to mimic and regenerate the human organoid system, and the LIFT technique has emerged as an efficient method for high resolution micropatterning and microfabrication of biomaterials and cells due to its capability of creating precise, controlled microdroplets. In this research, the parameters for an effective femtosecond laser-based LIFT process for 3D bioprinting of collagen biomaterial were studied. Different concentrations of collagen I solutions were tested to find out the right concentration that can be transferred through the …

Investigation Of Cell Behavior In 3d Printed Lumen Structures For Capillary Regeneration, Victoria Jade Perez

Theses and Dissertations

This thesis work successfully generated a coaxial printing setup and process to generate 3D printed tubules. A dual syringe pump mechanism was developed to print tubular structures to investigate cell behavior with a goal to generate future capillary beds. The mechanism involves the use of collagen–alginate tubules and the use of EDTA to increase the porosity of the tubule structures to study their interaction with media and incubation techniques as well as behavior and morphology. Tubules of 1.7% sodium alginate and 0.4% collagen I reacting with 3.2% CaCl2 solution proved to be more stable in both the printing process and …

Experimental Study Of Graphene Energy Absorbing Layer In Femtosecond Infrared Laser Assisted Bioprinting, Chaoran Dou

Theses and Dissertations

The bioprinting, as a new branch of additive manufacturing, already shown its potential in organ transplantation, medicine develop and personalized medicine. There are three types of bioprinting: droplet based bioprinting, extrusion based bioprinting and laser assisted bioprinting (LAB). Compared with other types of bioprinting, the LAB is the only bioprinting method that does not have a nozzle, which gives it the ability to print high viscosity bioink. The LAB also enjoys higher resolution and cell viability than other methods.

The bubble/jet formation process is the most important process for the LAB, which deposits the bioink to the substrate. To utilize …

Design And Testing Of A Modular Laser System With Laser Machining And Bioprinting Capabilities, Jesus Gonzalez

Theses and Dissertations

This thesis introduces the applications of ultrafast lasers while focusing on their bioprinting capabilities. A custom system was designed to utilize a femtosecond laser for 2D and 3D applications. The characteristics of lasers allow them to be efficiently used in machining, 3D printing, research, and more. Their power and precision allow them to be used for delicate work such as bioprinting. Bioprinting is a field holding great potential to benefit society. Studies conducted over the years prove its usefulness in printing cells and biomaterials, while showing there is still much to improve upon before being able to print fully functioning …