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Baseline Characterization Of Semen From Philippine Native Chickens: Banaba, Joloano And Paraoakan, Darcy Pamulaklakin, Carla Alilie Junsay, Consuelo Amor Estrella, Percival Sangel

The Philippine Agricultural Scientist

The study aimed to characterize and establish baseline data on the quantity and quality of fresh avian semen utilizing a sample of Philippine native roosters. Twenty-two heads each were acquired from breeds Banaba, Joloano, and Paraoakan. The baseline data analysis revealed significant variations in semen volume among different genetic groups, with Banaba displaying the highest volume, followed by Joloano and Paraoakan. Banaba also exhibited the highest semen pH value of 7.27 when compared to the other two genetic groups (P < 0.05). Across various genetic groups of native chickens, the majority of semen samples demonstrated a thick and creamy consistency and color (42.6%), followed by thin and creamy (32.9%), with a statistical significance of P < 0.05. Furthermore, variations in sperm concentration were observed among genetic groups, with Paraoakan having the highest sperm concentration, followed by Banaba and Joloano (P < 0.05). When the semen from Banaba and Joloano native roosters were extended in Ringer’s solution, it exhibited good motility, with more than 50% of spermatozoa in motion, even after six hours of extension. In contrast, the pooled extended semen from Paraoakan native roosters displayed poor motility, particularly after eight hours of extension (P < 0.05). The study also showed that storage temperatures did not significantly affect all the sperm quality parameters of Banaba and Joloano. However, for Paraoakan, samples stored at low temperatures of 5 – 10ºC had significantly higher percent motile and progressive sperm, and lower percent static sperm than those stored at room temperature (22 – 25ºC) (P < 0.05).

Standards In Semen Examination: Publishing Reproducible And Reliable Data Based On High-Quality Methodology, Lars Björndahl, Christopher L R Barratt, David Mortimer, Ashok Agarwal, Robert J Aitken, Juan G Alvarez, Natalie Aneck-Hahn, Stefan Arver, Elisabetta Baldi, Lluís Bassas, Florence Boitrelle, Riana Bornman, Douglas T Carrell, José A Castilla, Gerardo Cerezo Parra, Jerome H Check, Patricia S Cuasnicu, Sally Perreault Darney, Christiaan De Jager, Christopher J De Jonge, Joël R Drevet, Erma Z Drobnis, Stefan S Du Plessis, Michael L Eisenberg, Sandro C Esteves, Evangelini A Evgeni, Alberto Ferlin, Nicolas Garrido, Aleksander Giwercman, Ilse G F Goovaerts, Trine B Haugen, Ralf Henkel, Lars Henningsohn, Marie-Claude Hofmann, James M Hotaling, Piotr Jedrzejczak, Pierre Jouannet, Niels Jørgensen, Jackson C Kirkman Brown, Csilla Krausz, Maciej Kurpisz, Ulrik Kvist, Dolores J Lamb, Hagai Levine, Kate L Loveland, Robert I Mclachlan, Ali Mahran, Liana Maree, Sarah Martins Da Silva, Michael T Mbizvo, Andreas Meinhardt, Roelof Menkveld, Sharon T Mortimer, Sergey Moskovtsev, Charles H Muller, Maria José Munuce, Monica Muratori, Craig Niederberger, Cristian O'Flaherty, Rafael Oliva, Willem Ombelet, Allan A Pacey, Michael A Palladino, Ranjith Ramasamy, Liliana Ramos, Nathalie Rives, Eduardo Rs Roldan, Susan Rothmann, Denny Sakkas, Andrea Salonia, Maria Cristina Sánchez-Pozo, Rosanna Sapiro, Stefan Schlatt, Peter N Schlegel, Hans-Christian Schuppe, Rupin Shah, Niels E Skakkebæk, Katja Teerds, Igor Toskin, Herman Tournaye, Paul J Turek, Gerhard Van Der Horst, Monica Vazquez-Levin, Christina Wang, Alex Wetzels, Theodosia Zeginiadou, Armand Zini

Student and Faculty Publications

Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility of scientific publications. This is even more important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, and the International Standard ISO 23162:2021. With these tools, we propose that authors should be instructed to follow these laboratory methods in order to publish studies in peer-reviewed journals, preferable by using a checklist as suggested in an Appendix to this article.

The Effect Of Method Of Collection And Number Of Sequential Ejaculates On Semen Characteristics Of Beef Bulls, A. R. Hartman, M. L. Butler, S. K. Tucker, N. M. Goodenow, J. M. Bormann, D. M. Grieger

Kansas Agricultural Experiment Station Research Reports

Objective:The objective of this study was to determine the effect of collection method and number of sequential ejaculates on beef bull ejaculate characteristics.

Study Description:Semen collection data from 2008 to 2018 was obtained from the Kansas Artificial Breeding Services Unit and consisted of 11,642 ejaculates from 906 bulls. Bulls were collected twice weekly on Mondays and Thursdays with an artificial vagina. Bulls not receptive to the artificial vagina were subject to electro-ejaculation. A single technician was responsible for all pre-freeze and post-thaw semen analysis. Ejaculates were required to meet quality standards.

Results:Progressive motility before freezing was greater …

Quality And Fertility Of Extended Boar Semen After Prolonged Transport, Tomislav Barna, Jelena Apic, Aleksandar Milovanovic, Nevena Maksimovic, Aleksandar Masic, Marina Lazarevic, Milos Pavlovic

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106 , with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, …

Examination Of Methylation Sites For Forensic Age Determination From Semen, Christian Renwick, Sarah J. Seashols-Williams

Undergraduate Research Posters

Methylation Sensitive High-Resolution Melt (MS-HRM) is based on quantitating the melt curve from an experimental sample against a standard of known methylation levels. Whereas most applications of age prediction using methylation markers are based upon pyrosequencing or SNaPshot technologies, these analysis methods are both cost and instrumentation prohibitive. This study sought to use to the varied methylation status of the ELOVL2 and FHL2 alleles, both having known correlation with age (Hamano et. al.), in a labor and time efficient manner to develop an age prediction model. A non-linear regression and standard curve was compiled from the methylation status in a …

Allelic Variation In Cxcl16 Determines Cd3+ T Lymphocyte Susceptibility To Equine Arteritis Virus Infection And Establishment Of Long-Term Carrier State In The Stallion, Sanjay Sarkar, Ernest Bailey, Yun Young Go, R. Frank Cook, Ted Kalbfleisch, John E. Eberth, R. Lakshman Chelvarajan, Kathleen M. Shuck, Sergey Artiushin, Peter J. Timoney, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of horses and other equid species. Following natural infection, 10–70% of the infected stallions can become persistently infected and continue to shed EAV in their semen for periods ranging from several months to life. Recently, we reported that some stallions possess a subpopulation(s) of CD3⁺ T lymphocytes that are susceptible to in vitro EAV infection and that this phenotypic trait is associated with long-term carrier status following exposure to the virus. In contrast, stallions not possessing the CD3⁺ T …

Consolidación De Sistemas De Selección De Las Razas Criollas Y Producción De Material Genético De Animales Elite (Año 2), Objetivo: Desarrollar Un Plan De Mejoramiento Genético En Razas Criollas, Mediante El Uso Material Germinal De Individuos Superiores Seleccionados Dentro De Las Poblaciones De Referencia Evaluadas, Rodrigo Rolando Rocha Sanabria

Zootecnia

La biotecnología reproductiva ha avanzado con el pasar de los años, con el fin de preservar y mejorar ejemplares representativos de las diferentes razas mediante métodos tales como: congelación de semen convencional y sexado, producción de embriones convencional y fertilización in vitro FIV, entre otros. En la Corporación Colombiana de Investigación Agropecuaria (CORPOICA), se inició con la producción de embriones por el método convencional con animales de las razas Romosinuano y Blanco Oreginegro (BON), realizando cruzamientos con diferentes razas tales como: Angus, Piemontese y Holstein. La comercialización del producto final, se da a través de un catálogo con información obtenida …

Dose-Dependent Effects Of Tamsulosin On The Ejaculation And Semen Quality In Bucks, Sakdichod Kimsakulvech, Peerasak Suttiyotin, Anuchai Pinyopummin

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effect of tamsulosin (TAM) dosages on ejaculation and semen quality. Two sets of Latin square experiment design were applied to 6 bucks receiving normal saline (CON), TAM 134.8 nM/kg (LTAM), or 269.7 (HTAM) nM/kg at 1-week intervals. Semen collection, libido, and ejaculatory scoring were undertaken at 3, 6, 9, 12, and 24 h after injection. Heart rate and body temperature were measured before administration and at 30 min, and before semen collection. The results showed that libido score and heart rate were not affected by treatments. Ejaculatory suppression was observed in …

The Effect Of Oxytocin And Prostaglandin Hormones Added Tosemen On Stallion Sperm Quality, Çi̇ğdem Çebi̇ Şen, Ergun Akçay

Turkish Journal of Veterinary & Animal Sciences

Prostaglandin F2α (PGF2α) and oxytocin have been used to improve reproductive performance in many mammalian species, including humans. The aim of the present study was to determine their effects on sperm quality when extended stallion semen was enriched with PGF2α or oxytocin. In this study, 16 healthy adult stallions, each 15-22 years old, were used. Five ejaculates from each stallion were collected with an artificial vagina during breeding season. Thereafter, gel-free semen was divided into 7 aliquots and diluted with an INRA96 semen extender. The following different treatments were evaluated: 3 with only PGF2α (10, 20, and 40 μg/mL) and …

Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky

Turkish Journal of Veterinary & Animal Sciences

Improving sire reproductive performance in artificial insemination (AI) programs and maximizing the use of sires with valuable genomes are high on the list of priorities for livestock breeders in the United States and worldwide. While fixed-time AI and accurate estrous detection have already ameliorated the reproductive management of cows and sows, substantial gains remain to be made in the selection and utilization of male animals for field AI. In addition to better, more objective evaluation of breeding soundness and semen quality, numbers of spermatozoa per AI dose could be reduced and numbers of AI doses per collection could be increased …

Semen Quality In White Leghorn Chicken Selected For Egg Production Traits, Shanmugam Murugesan, Niranjan Matam, Rakesh Kulkarni, Tarun Kumar Bhattacharya, Rudranath Chatterjee

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to evaluate the effect of selection for egg production traits in White Leghorn chicken based upon the semen quality. Males of 3 pure lines of White Leghorn, namely IWH, IWI, and IWK, along with a pedigree random-bred control (IWC) population, were selected and housed in individual cages. The semen collected from individual birds was evaluated for different physical and biochemical parameters. There was significant (P =< 0.013) difference in semen quality parameters studied between the lines. High egg-producing lines IWH and IWI had poor semen quality in comparison with that of the control line (IWC). Thus, it can be concluded that selection for higher egg production affects the semen quality of males in the selected lines.

Effect Of Extenders, Postdilution Intervals, And Seasons On Semen Quality In Dairy Goats, Muhammad Subhan Qureshi, Daulat Khan, Anila Mushtaq, Shoaib Sultan Afridi

Turkish Journal of Veterinary & Animal Sciences

The breeding of female dairy goats with good quality sires has been a problem in rural areas and artificial insemination (AI) provides an answer to this issue. To develop an AI model, a total of 9 bucks were purchased from a local market. Semen was collected in an artificial vagina, the libido was noted, and the semen was evaluated for its physical characteristics. The libido, semen volume, mass motility, individual motility, and sperm concentration were 2.94 ± 0.25 (scale of 1-3), 0.79 ± 0.59 mL, 4.34 ± 0.93 (scale of 1-5), 55.01 ± 32.59%, and 1027 ± 406 million spermatozoa/mL, …

Effects Of Osteopontin Single Nucleotide Polymorphisms On Bull Semen Quality, Chance L. Williams

Graduate Theses and Dissertations

Two groups of bulls were utilized to evaluate if single nucleotide polymorphisms (SNP) in the Bos taurus OPN gene (GenBank accession # AY878328.1) promoter region were related to bull semen quality variables as evaluated by computer assisted semen analysis (CASA). Group 1 consisted of 19 Angus and Balancer bulls, ranging in age from 5 to 8 years, which were collected weekly during a 2-month period (July 15, 2010 through September 19, 2010). Group 2 consisted of 16 Brahman influenced (1/8 to 3/16 Brahman) bulls, mean age = 1.1 ± 0.1 year, that were collected monthly for a 7 month period …

Effect Of Ejaculation Frequency On Spermatozoa Survival In Diluted Semen From Pleven Blackhead Rams, Stanimir Yotov, Ivan Fasulkov, Nasko Vassilev

Turkish Journal of Veterinary & Animal Sciences

The effect of ejaculation frequency on spermatozoa survival in diluted Pleven Blackhead ram semen was investigated. Thirty-two ejaculates were examined. From each ram 4 ejaculates were collected at 10-min intervals in the morning and in the afternoon. Semen parameters, namely, color, density, transparency, volume, concentration, motility, and abnormal sperm, for each ejaculate were determined. The sperm motility was assessed from the moment of dilution up to 60 min at 10-min intervals, then again at 90 min and 120 min. The semen characteristics were determined by means of "Motic Image Plus" equipment and the data were processed statistically. Sperm volume and …

Influence Of Pgf_{2\Alpha} On Semen Quality And Libido In Holstein Bulls, Reza Masoumi, Armin Towhidi, Ardsher N. Javaremi, Habib Nabizadeh, Mehdi Zhandi

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of Cloprostenol (PGF_{2\alpha} analogue) on semen quality and libido in Holstein bulls. Ten low libido Iranian Holstein bulls were randomly allocated to 2 groups and received Cloprostenol (n = 5) or saline (n = 5) 30 min prior to the semen collection 2 days per week for 2 months. Reaction time was significantly decreased in the treatment group. Duration of ejaculation was significantly increased in the treatment group. Semen volume and sperm concentration were higher in treated bulls when compared with controls. The percentage of morphologically normal sperm cells, percentage …

Repeated Sampling Affects Tree Swallow Semen Characteristics, Michael Lombardo, M.L. Green, P.A. Thorpe, M.R. Czarnowski, H.W. Power

Michael P Lombardo

Male Tree Swallows (Tachycineta bicolor) face intense sperm competition because mated pairs copulate frequently, extra-pair copulations are common, and females store sperm. We examined the effects of repeated sampling on the characteristics of Tree Swallow semen by manually expressing semen from 15 males immediately after capture (T₀) and then hourly for 4 h (T₁-T₄). The semen characteristics of individual males varied in response to repeated sampling. The total number of sperm cells we obtained from each male over the 4-h sampling period varied from 10⁴-10⁷. Semen samples lacking …

Individual, Temporal, And Seasonal Variation In Sperm Concentration In Tree Swallows, Michael Lombardo, Armetris Forman, Matthew Czarnowski, Patrick Thorpe

Michael P Lombardo

We determined sperm concentrations in Tree Swallows (Tachycineta bicolor) by manually expressing semen samples from males during prelaying, egg-laying, incubation, and nestling periods. Sperm concentrations varied by orders of magnitude (0-109 sperm mL-1) among males. Sperm concentrations were highest during the incubation period and lowest during the prelaying period. None of the samples collected during the prelaying, egg-laying, and incubation periods were devoid of sperm. In contrast, 45% of samples collected during the nestling period lacked sperm. Sperm concentrations (1) did not vary over the course of the morning during prelaying, egg-laying, and incubation periods but significantly increased during the …

Mississippi Sandhill Crane Chicks Produced From Cryopreserved Semen, Anahid M. Pahlawanian, Megan L. Savoie, Vanessa Peery, Betsy L. Dresser, S. P. Leibo

Proceedings of the North American Crane Workshop

The Mississippi sandhill crane (MSC, Grus canadensis pulla), 1 of 6 subspecies of sandhill cranes, is classified as critically endangered and was placed on the United States' List of Endangered Fish and Wildlife in 1973. For 13 years starting in 1996, the Audubon Center for Research of Endangered Species (ACRES) and Freeport-McMoran Species Survival Center (SSC) in New Orleans have been contributing to the MSC Recovery Program. Through successful captive propagation of MSCs by use of natural breeding and artificial insemination, more than 150 chicks raised at SSC have been released into the wild population at the MSC National …

Prediction Of Postpubertal Reproductive Potential According To Prepubertal Body Weight, Testicular Size, And Testosterone Concentration Using Multiple Regression Analysis In Kıvırcık Ram Lambs, Özkan Elmaz, Serdal Di̇kmen, Ümi̇t Ci̇ri̇t, Hidir Demi̇r

Turkish Journal of Veterinary & Animal Sciences

The relationship between the prepubertal body weight, testicular size, testosterone concentration, and postpubertal reproductive function was investigated in Kıvırcık ram lambs. The body weight, testicular size, and testosterone concentration were measured every 20 days between 60 and 420 days of age. Semen was collected from the ram lambs at 7, 8, 9, 10, 11, 12, 13 and 14 months of age. Data obtained were analyzed by best subsets regression model. We determined that body weight, scrotal circumference, testicular length, and testosterone levels in the early prepubertal period (days 80, 100, and 120), when combined with each other, allow the determination …

Effect Of Low Temperature Thawing On The Motility And Fertility Of Cryopreserved Water Buffalo And Zebu Bull Semen, M. Anwar, S.M.H. Andrabi, A. Mehmood, N. Ullah

Turkish Journal of Veterinary & Animal Sciences

The study was conducted to evaluate the quality of cryopreserved buffalo and zebu semen thawed and held at low temperature. Progressive motility of frozen semen thawed in a 37 °C water bath for 45 s (control) was compared with that of semen held in ice water (3-5 °C) for 180 min. Semen collected from 3 buffalo and 2 zebu bulls was used for this purpose over 3 weeks (replicates). Fertility was compared after performing 28 inseminations with buffalo semen and 100 inseminations with zebu semen either after control thawing or thawing straws in ice water for 30-60 min. Progressive motility …

Determining The Efficacy Of A Commercially Available Post-Thaw Bovine Semen Sexing Kit, Erin Curry

All Theses

Swaying the bovine sex ratio is beneficial because dairy heifers are significantly more valuable than bull calves, whereas in the beef industry, bull calves are more desirable than females. Currently, there is no inexpensive, reliable, effective method for commercially separating X- and Y- bearing sperm. The objective of these experiments was to determine the efficacy of a commercially available post-thaw bovine semen sexing kit, HeiferPlusTM, which claims to sway the sex ratio in favor of female calves.
Three trials included the insemination of hyperstimulated cows with Control or HeiferPlusTM treated semen, non-surgical embryo collection on Day 7, and a novel …

Diluents For Cryopreservation Of Semen From Non-Woolly Llamas (Lama Glama), Franz Nicolas Bustos Fernández

Theses and Dissertations

The collection and preservation of llama semen is not a standardized procedure as it is in cattle. It has a number of complications that are mainly associated with the characteristics of the semen. In order to contribute to the knowledge of reproductive biology, we decided to evaluate the behavior of llama semen in relation to the different diluters in this study. This study was done at the National Germplasm Bank for Camelids which is part of the Ministry of Rural Development of Agriculture and Environment. The Ministry is located in the Agriculture Experiment Center of Condoriri which belongs to the …

Effect Of Fenbendazole On Turkey Semen Quality, M. R. Bakst, M. Kramer, J. Long

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fenbendazole (FBZ) is an anthelmintic recently approved to treat and control nematode infections in growing turkeys. When administered to growing turkeys there are no detrimental side effects. However, when we used FBZ to treat nematodes in mature breeder toms, we observed a decrease in semen quality and a subsequent precipitous decline in fertility to less than 20% within 6 wk of administration. An experiment was designed to determine the impact of FBZ administration on aspects of spermatogenesis and semen quality. We discovered that although sperm viability and concentration was not significantly affected by FBZ, this drug significantly reduced sperm mobility. …

In Vitro Effects Of Prostaglandin F_{2a} And Metamizol On The Motility Of Diluted Bull Semen, İzzet Karahan, Gaffari̇ Türk, Seyfetti̇n Gür

Turkish Journal of Veterinary & Animal Sciences

The purpose of the present study was to investigate in vitro effects of prostaglandin F_2 alpha (PGF_{2a}) and metamizol on the motility of diluted bull semen during short-term storage at 4 ºC. In this study, 3 healthy adult Brown-Swiss bulls, each 3 years old, were used. Semen samples were collected with an artificial vagina. Samples were taken from each bull twice a week over the course of 3 weeks (6 samples per bull). One millilitre of semen was removed from each sample for the determination of semen volume, mass activity, initial motility, sperm concentration, and abnormal sperm rate. The remainder …

In Vitro Effects Of Melatonin On Hyaluronidase Activity And Sperm Motility In Bull Semen, Sadetti̇n Tanyildizi, Tanzer Bozkurt, Osman Çi̇ftçi̇, Fati̇h Saki̇n

Turkish Journal of Veterinary & Animal Sciences

The effects of melatonin on hyaluronidase activity of semen and spermatozoa motility of Holstein bulls were investigation in vitro. Semen samples were divided into 5 equal parts and incubated (v/v) with melatonin at the doses of 0.5, 1, 2 and 4 mM for 60 min. Percentages of spermatozoa motility, morphological abnormality and hyaluronidase activity of semen were determined at 5, 10, 15, 20, 30 and 60 min during incubation. Results show that melatonin caused a significant (P < 0.001) decrease in the spermatozoa motility, while causing a significant increase in the hyaluronidase activities of the semen. However, there was no significant difference in the percentages of abnormal spermatozoa when compared with control samples. Doses of 0.5, 1, 2 and 4 mM of melatonin in bull semen decreased the percentage of spermatozoa motility in a time- and dose-dependent manner. The mean percentages of abnormal spermatozoa were not affected by melatonin treatment. However, it caused an increased hyaluronidase activity of bull semen. In conclusion, the existence of melatonin in bull semen has a significant effect on fertilization ability by affecting spermatozoa motility and hyaluronidase activity.

Some Reproductive Parameters And Seminal Plasma Constituents In Relation To Season In Akkaraman And Awassi Rams, Mustafa Gündoğan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to describe seasonal changes in scrotal circumference, sperm motility, sperm concentration, percentage of abnormal spermatozoa and biochemical and enzymatic properties of seminal plasma for 9 months in 10 mature fat-tailed Akkaraman and Awassi breed rams. Scrotal circumference and semen were evaluated monthly from rams and seminal plasma separated from semen to study it. Scrotal circumference, sperm motility and sperm concentration were higher during autumn months. Furthermore, autumn months showed moderate percentage of abnormal spermatozoa. The biochemical analyses of ram seminal plasma indicated that the highest values of total protein, albumin (A), globulin (G), total …

In Vitro Effects Of Linamarin, Amygdalin And Gossypol Acetic Acid On Hyaluronidase Activity, Sperm Motility And Morphological Abnormality In Bull Sperm, Sadetti̇n Tanyildizi, Tanzer Bozkurt

Turkish Journal of Veterinary & Animal Sciences

In vitro effects of various doses of linamarin, amygdalin and gossypol acetic acid on sperm hyaluronidase activity and the percentages of motile spermatozoa and morphology were investigated in bull sperm. Holstein bulls (n = 12) aged between 2 and 3 were used in the study. Semen samples were collected from each animal 3 times and used for incubation. The samples were divided into 5 equal parts and mixed (v/v) with linamarin at doses of 0.5, 0.75, 1 and 2 µM, with amygdalin at doses of 0.4, 0.8, 1 and 2 µM and with gossypol acetic acid at doses of 2.5, …

Cryopreservation Of Mirror Carp Semen, Ergun Akçay, Yusuf Bozkurt, Selçuk Seçer, Necmetti̇n Teki̇n

Turkish Journal of Veterinary & Animal Sciences

The effects of different extenders and cryoprotectants on the viability and fertilizing ability of frozen spermatozoa from the mirror carp, Cyprinus carpio L. (1758), were investigated. Semen was collected from anesthesized males by the abdominal massage method. Having determined the main spermatological properties (volume, motility, duration of motility, total spermatozoa number, concentration and pH), the pooled ejaculates were diluted with 3 extenders containing different cryoprotectants (15% DMSO, 15% DMA, 15% Glycerol) individually. One part semen was added to 3 parts extender. The diluted semen was packaged in 0.5 ml straws and left to equilibrate for 45 min at 4 °C. …

The Effect Of Ascorbic Acid On The Freezability Of Ram Semen Diluted With Extenders Containing Different Proportions Of Glycerol, Mustafa Sönmez, Eşref Demi̇rci̇

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of ascorbic acid on the freezability and spermatological characteristics of ram semen diluted with Tris-glucose-egg yolk based extenders containing different proportions of glycerol. Six Akkaraman rams were used. Semen was collected by electroejaculator once every 3 days. The spermatological characteristics of the collected semen samples were determined. Pooled and diluted semen was cooled slowly to + 4 ºC over 2 h. Cooled semen was divided into 20 groups. Each of the 20 semen groups was diluted at a 1:1 ratio with one of the extender groups containing different proportions of glycerol (0%, …

The Effect Of Age On Spermatological Properties In Rainbow Trout (Oncorhynchus Mykiss W., 1792), Necmetti̇n Teki̇n, Selçuk Seçer, Ergun Akçay, Yusuf Bozkurt, Sezer Kayam

Turkish Journal of Veterinary & Animal Sciences

At the end of the spawning season, semen was collected without anesthesia by abdominal massage from 37 rainbow trout according to different age groups. In collected semens, color, volume, motility, activity time (duration of motility), concentration, total spermatozoa and pH were recorded. Furthermore, body weight and body length were measured and correlations beetween spermatological properties and these parameters were assessed. In the 1-2 years age group, volume (ml), motility (%), activity time (s), concentration (x 10^9 sp/ml), total spermatozoa (per ejaculate) and pH were 9.7, 81.0, 79.0, 8.97, 87.0 and 7.20 respectively; in the 2-3 years age group, these values …