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Oncogenic Transformation By Herpes Simplex Virus, James Christian Burns

Theses and Dissertations

Many lines of evidence exist associating herpes simplex virus (HSV) with the development of carcinoma. Data from human studies includes seroepidemiologic studies of carcinoma patients and the localization of viral markers in human cancers. Experimental studies include in vitro transformation of cultured cells and viral induced alterations of host DNA. Much of this evidence is anecdotal or associative in nature and does not prove a cause and effect. The purpose of this research was to investigate the oncogenic potential of HSV type 2 (HSV-2) in vivo and in vitro.

An in vivo mouse model for lib carcinogenesis was designed to …

The Responsiveness Of Murine Tumors To Maleic Vinyl Ether, Paal Christian Klykken

Theses and Dissertations

Studies were undertaken to characterize the immune status of mice bearing the Lewis lung carcinoma (LLC) and to maximize the cure rate of LLC and Madison l09 carcinoma (Ml09) bearing mice by employing the immunomodulator maleic vinyl ether (MVE) in combination with surgery or radiotherapy.

The pattern of immunodeficiency in mice bearing the LLC appeared to be in contrast to most studies. LLC mice with a minimal tumor burden were found to have a diminished ability to phagocytize sheep erythorcytes (SRBC) or elicit an antibody response to the same antigen. Vascular clearance and phagocytic uptake of ⁵¹Cr-labeled SRBC into …

The Effects Of Streptozotocin-Induced Diabetes On Responses To Opiates And Other Centrally-Acting Pharmacologic Agents, Glenn Stuart Simon

Theses and Dissertations

Diabetes mellitus affects millions of people. Although diabetics can lead relatively normal lives, all treatments for the disease are symptomatic and not curative. The purpose of this investigation was to determine whether the sensitivity to opiates and other selected centrally-acting drugs in animals is altered by streptozotocin (STZ)-induced diabetes. A second objective was to determine which aspect of the diabetic syndrome primarily was responsible for the altered sensitivity. Other experiments were performed in an attempt to elucidate the mechanism whereby this altered sensitivity occurred.

STZ-induced diabetes or dextrose-induced transient hyperglycemia did not have a significant effect on the duration of …

The Biological And Structural Characterization Of Transferable Bacteroides R Plasmids, Rodney A. Welch

Theses and Dissertations

In this literature review I will briefly describe the general biology of transferable antibiotic resistance in bacteria, the genetic elements involved (plasmids) and several specific plasmid associated phenotypes. This will be followed with a review of the general biology of Bacteroides, the information known concerning its plasmid reservoir and finally a review of the information documenting the emergence of transferable clindamycin resistance in Bacteroides. The latter aspect represents the central focus of research described in this thesis.

Antiviral Activity Of Peritoneal Exudate Cells Of Mice Against Infection With Herpes Simplex Virus Type 2, Margaret Byrd Mcgeorge

Theses and Dissertations

Within the past decade, there has been increasing evidence which suggests that herpes simplex virus type two (HSV-2) is associated with cervical carcinoma. Because cancer of the cervix ranks as the second most common malignant disease of women, with 35 thousand new cases and 10,000 deaths per year, further investigation is warranted to determine if there is a causal relationship (Goldberg, 1976). Currently, most of the evidence which correlates HSV-2 with cancer of the cervix has been, for the most part, indirect: (i) there is an increased incidence of cervical anaplasia in women with cytologically detectable genital herpes infection, (ii) …

Characterization Of The Bursicon-Mediated Pathway For Membrane Permeability Enhancement In The American Cockroach, Periplaneta Americana, David Alan Compton

Theses and Dissertations

The presence of an adenyl cyclase system within the haemocyte of Periplaneta americana has been confirmed by previous investigations, with corresponding experiments using dibutyryl cyclic AMP to show enhancement of 14c-tyrosine uptake into the cuticle linked to stimulation of the sclerotization process.

In the present studies, American cockroaches held at depressed temperatures were treated with bursicon, revealing an increase in haemocyte cyclic AMP concentrations. The levels declined with progression of time, probably resulting from enzymatic hydrolysis.

When animals which had been held at room temperature were treated with bursicon, the levels of haemocyte cyclic AMP were lowered, with a declination …

Intravenous Infection Of Mice With Naegleria Fowleri, Richard G. May

Theses and Dissertations

Primary amebic meningoencephalitis is a fatal disease of man caused by the free-living ameboflagelate Naegleria fowleri. In general, the victims have been active, healthy, young adults with a recent history of swimming or other fresh water-related activity. Infection with N. fowleri is apparently by way of nasal introduction of amebae-containing water. After nasal installation, electron microscopic and histopathologic studies with experimental animals reveal that amebae reside in the olfactory mucosa and then invade and migrate through the submucosal structures into the nerve plexuses. Amebae pass through pores of the cribiform plate and into the subarachnoid space. Subsequently, amebae invade the …