Digital Commons Network^™

Characterization Of Antimicrobial Susceptibility Profile Of Biofield Treated Multidrug-Resistant Klebsiella Oxytoca, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Engaging Youth In Lifelong Outdoor Adventure Activities Through A Nontraditional Public School Physical Education Program, Keri Schwab, Daniel L. Dustin

Keri Schwab

Engaging Youth in Lifelong Outdoor Adventure Activities through a Nontraditional Public School Physical Education Program

Tsu Faculty Publication Database, David Owerbach

David Owerbach

THE TSU faculty publication database is for the years 2012-2014. The database was constructed by the Office of Research and was last updated on November 20, 2014.

Longitudinal Transcriptomic Dysregulation In The Peripheral Blood Of Transgenic Huntington’S Disease Monkeys, Jannet Kocerha, Yuhong Liu, David Willoughby, Kumaravel Chidamparam, Joseph Benito, Kate Nelson, Yan Xu, Tim Chi, Heidi Engelhardt, Sean Moran, Shang-Hsun Yang, Shi-Hua Li, Xiao-Jiang Li, Katherine Larkin, Adam Neumann, Heather Banta, Jinjing Yang, Anthony W. S. Chan

Jannet Kocerha

Background: Huntington’s Disease (HD) is a progressive neurodegenerative disorder caused by an expansion in the polyglutamine (polyQ) region of the Huntingtin (HTT) gene. The clinical features of HD are characterized by cognitive, psychological, and motor deficits. Molecular instability, a core component in neurological disease progression, can be comprehensively evaluated through longitudinal transcriptomic profiling. Development of animal models amenable to longitudinal examination enables distinct disease-associated mechanisms to be identified.

Results: Here we report the first longitudinal study of transgenic monkeys with genomic integration of various lengths of the human HTT gene and a range of polyQ repeats. With this unique group …

Physical Properties Of Yogurt Fortified With Various Commercial Whey Protein Concentrates, Isabelle Sodini, John Montella, Phillip S. Tong

Phillip S. Tong

The effects of whey protein concentrates on physical and rheological properties of yogurt were studied. Five commercial whey protein concentrates (340 g kg−1 protein nominal) were used to fortify milk to 45 g protein kg−1. Fermentation was performed with two different starters (ropy and non-ropy). Resulting yogurts were compared with a control yogurt enriched with skim milk powder. The water-holding capacity of the yogurt fortified with skim milk powder was 500 g kg−1 and ranged from 600 to 638 g kg−1 when fortified with whey protein concentrates. Significant rheological differences have been noticed between the yogurts fortified with different whey …

Advances In Profiling Of Noncoding Rnas In Neurological Disease, Murray J. Cairns, Jannet Kocerha

Jannet Kocerha

No abstract provided.

Expression Of Fused In Sarcoma Mutations In Mice Recapitulates The Neuropathology Of Fus Proteinopathies And Provides Insight Into Disease Pathogenesis, Christophe Verbeeck, Mariely Dejesus-Hernandez, Carolina Ceballos-Diaz, Jannet Kocerha, Todd Golde, Pritam Das, Rosa Rademakers, Dennis W. Dickson, Thomas Kukar

Jannet Kocerha

Background: Mutations in the gene encoding the RNA-binding protein fused in sarcoma (FUS) can cause familial and sporadic amyotrophic lateral sclerosis (ALS) and rarely frontotemproal dementia (FTD). FUS accumulates in neuronal cytoplasmic inclusions (NCIs) in ALS patients with FUS mutations. FUS is also a major pathologic marker for a group of less common forms of frontotemporal lobar degeneration (FTLD), which includes atypical FTLD with ubiquitinated inclusions (aFTLD-U), neuronal intermediate filament inclusion disease (NIFID) and basophilic inclusion body disease (BIBD). These diseases are now called FUS proteinopathies, because they share this disease marker. It is unknown how FUS mutations cause disease …

G73-73 Sweet Potatoes (Revised June 1992), R.E. Neild, Laurie Hodges

Laurie Hodges

Varieties, requirements and culture, harvesting and storage of sweet potatoes. The sweet potato, a member of the morning glory plant family, is native to tropical America. It is an important food plant in warmer regions of the world and is adapted to southeastern Nebraska. Sweet potatoes may be boiled, baked, fried, or candied. They have high food value. Varieties with deeply yellow colored roots are a good source of vitamin A.

Nf97-344 Weights And Measures Guidelines For Sales At Farmers' Markets, Roadside Stands And Other Commercial Outlets, Laurie Hodges

Laurie Hodges

This NebFact focuses on the sale of fresh produce at farmers' markets, roadside stands, and other seasonal outlets.

Nf91-50 Bee Pollination Of Cucurbit Crops, Laurie Hodges, Fred Baxendale

Laurie Hodges

This NebFact discusses bee pollination of cucurbit crops.

G00-1778 Field Windbreaks, James Brandle, Laurie Hodges

Laurie Hodges

gricultural producers face many challenges as they try to balance efficient production systems with increasing environmental demands. For these systems to be successful, they must optimize the balance between inputs and final production. Field windbreaks are one way to increase yields while at the same time reducing inputs and improving both environmental quality and production efficiency. Windbreaks reduce wind speed and alter the microclimate in sheltered areas. Field windbreaks reduce wind erosion and the damage to crops caused by wind-blown soil. They improve water use efficiency, reduce risks associated with drought, and manage blowing snow.

Field windbreaks provide positive economic …

Nf92-69 Selected Vegetable Cultivars For Nebraska, Susan Schoneweis, Laurie Hodges

Laurie Hodges

This NebFact provides information about cultivars of garden vegetables available to grow in Nebraska.

G90-993 Basic Cultural Practices For Commercial Production Of Green (Snap) Beans, Laurie Hodges

Laurie Hodges

This NebGuide discusses cultivar selection, production, pest management and packing of commercial green beans. Cultivars Selection of cultivars depends on intended use or market. "Processor" cultivars are low in fiber and are best for home garden and roadside markets as well as processing. These include the various Bush Blue Lake selections (BBL 47, BBL 92, BBL 274) and others such as Early Gallatin, Eagle, Slenderwhite, Labrador, Flo, and Peak. The "fresh market" or "shipping" beans have high fiber to maintain quality during long distance shipping. These include Strike, Podsquad, Blazer, Triumph, Atlantic, Gatorgreen, Greencrop, Sprite, and many others. Cultivars with …

Nf91-43 Blossom End Rot In Tomatoes, Laurie Hodges, Don Steinegger

Laurie Hodges

This NebFact discusses the causes, prevention and treatment of blossom end rot in tomatoes.

G80-496 Tomatoes In The Home Garden, Laurie Hodges, Dale T. Lindgren, Susan Schoneweis

Laurie Hodges

This NebGuide outlines tomato rearing practices, cultivars and possible pest, disease and weed control problems.

Tomatoes come in a wide range of fruit colors, sizes, shapes and maturities. Ripe tomatoes may be red, yellow, orange, pink or even green. Shapes vary from globe or round to slightly flattened, pear-like or cherry-sized. Often consumers complain tomatoes purchased in grocery stores are lacking in flavor or have tough skin. In a home garden, you can grow the tomatoes you prefer, including a wide selection of fruit colors, flavors, textures and sizes. Although rumored, there is no direct link between fruit acidity and …

Nf96-313 Guides For Development Of A Farmers Market, Laurie Hodges

Laurie Hodges

This NebFact contains a list of resources for starting and running a farmers' market.

Nf94-127 Growing Seedless (Triploid) Watermelons, Laurie Hodges

Laurie Hodges

This NebFact has information on growing seedless watermelons.

Nf99-404 Income Generation Using Alternative Crops, Roger D. Uhlinger, Laurie Hodges

Laurie Hodges

This NebFact examines considerations for utilizing alternative crops for income generation.

G74-108 Wilts Of Cucurbits (Revised October 1994), James R. Steadman, David L. Keith, Laurie Hodges

Laurie Hodges

Discussion covers the symptoms, disease cycles, and control measures for bacterial and Fusarium wilts of cucurbits, including cucumbers, cantaloupe, watermelons, squash, and pumpkins. Wilt caused by squash vine borer also is covered.

Nf92-104 Post-Harvest Handling Of Commercial Vegetable Crops -- Resource List, Laurie Hodges

Laurie Hodges

This NebFact gives a resource list for post-harvest handling of commercial vegetable crops.

Nf91-51 Kale: The "New" Old Vegetable, Laurie Hodges

Laurie Hodges

This NebFact discusses kale.

Nf92-53 European Corn Borer Injury To Peppers, Frederick P. Baxendale, Laurie Hodges

Laurie Hodges

This NebFact discusses European corn borer injury to peppers.

Nf97-353 What Is Quality. . . .In A Tomato?, Laurie Hodges

Laurie Hodges

This NebFact examines the many factors affecting tomato quality.

Nf96-274 Seed Sources For Commercial Vegetable Production, Laurie Hodges

Laurie Hodges

This NebFact has addresses of distributors of seeds for commercial vegetable production.

The Path To Microrna Therapeutics In Psychiatric And Neurodegenerative Disorders, Anthony W. S. Chan, Jannet Kocerha

Jannet Kocerha

The microRNA (miRNA) class of non-coding RNAs exhibit a diverse range of regulatory roles in neuronal functions that are conserved from lower vertebrates to primates. Disruption of miRNA expression has compellingly been linked to pathogenesis in neuropsychiatric and neurodegenerative disorders, such as schizophrenia, Alzheimer’s disease, and autism. The list of transcript targets governed by a single miRNA provide a molecular paradigm applicable for therapeutic intervention. Indeed, reports have shown that specific manipulation of a miRNA in cell or animal models can significantly alter phenotypes linked with neurological disease. Here, we review how a diverse range of biological systems, including Drosophila, …

G73-46 Hessian Fly On Wheat, John E. Foster, Gary L. Hein

John E. Foster

This NebGuide discusses the life cycle, control and prevention of the Hessian fly. Plant-safe dates and resistant wheat varieties are also examined. The Hessian fly, Mayetiola destructor (Say), is not native to the United States, but was probably introduced by Hessian soldiers during the Revolutionary War. This insect was given its common name by Americans because of its damage on Long Island in 1779. The pest has become distributed throughout the United States wheat production areas since then. The Hessian fly belongs to the family of insects known as gall midges (Diptera: Cecidomyiidae), a group noted for their habit of …

Altered Microrna Expression In Frontotemporal Lobar Degeneration With Tdp-43 Pathology Caused By Progranulin Mutations, Jannet Kocerha, Naomi Kouri, Matt Baker, Nicole Finch, Mariely Dejesus-Hernandez, John Gonzalez, Kumaravel Chidamparam, Keith A. Josephs, Bradley F. Boeve, Neill R. Graff-Radford, Julia Crook, Dennis W. Dickson, Rosa Rademakers

Jannet Kocerha

Frontotemporal lobar degeneration (FTLD) is a progressive neurodegenerative disorder that can be triggered through genetic or sporadic mechanisms. MicroRNAs (miRNAs) have become a major therapeutic focus as their pervasive expression and powerful regulatory roles in disease pathogenesis become increasingly apparent. Here we examine the role of miRNAs in FTLD patients with TAR DNA-binding protein 43 pathology (FTLD-TDP) caused by genetic mutations in the progranulin (PGRN) gene.

Results

Using miRNA array profiling, we identified the 20 miRNAs that showed greatest evidence (unadjusted P < 0.05) of dysregulation in frontal cortex of eight FTLD-TDP patients carrying PGRN mutations when compared to 32 FTLD-TDP patients with no apparent genetic abnormalities. Quantitative real-time PCR (qRT-PCR) analyses provided technical validation of the differential expression for 9 of the 20 miRNAs in frontal cortex. Additional qRT-PCR analyses showed that 5 out of 9 miRNAs (miR-922, miR-516a-3p, miR-571, miR-548b-5p, and miR-548c-5p) were also significantly dysregulated (unadjusted P < 0.05) in cerebellar tissue samples of PGRN mutation carriers, consistent with a systemic reduction in PGRN levels. We developed a list of gene targets for the 5 candidate miRNAs and found 18 genes dysregulated in a reported FTLD mRNA study to exhibit anti-correlated miRNA-mRNA patterns in affected cortex and cerebellar tissue. Among the targets is brain-specific angiogenesis inhibitor 3, which was recently identified as an important player in synapse biology.

Conclusions

Our study suggests that miRNAs may contribute to the pathogenesis of FTLD-TDP caused by PGRN mutations and …

Genetic Admixture In Multidimensional Niche Space: Asymmetrical Niche Similarity Promotes Gene Flow In Armadillos (Dasypus Novemcinctus), Maria Arteaga, John Mccormack, Luis Eguiarte, Rodrigo Medellín

John E. McCormack

We unite genetic data with a robust test of niche divergence to test the hypothesis that patterns of gene flow between two lineages of the nine-banded armadillo are influenced by their climatic niches. We collected Geographical Information System (GIS) data on climate using locality information from 111 individuals from two lineages that had associated genetic material. We tested whether niches of these lineages were more conserved or divergent than the background environments of their geographic ranges and found evidence for niche conservatism on two axes and no evidence for divergence on any axis. To address the role of niche similarity …

Drosophila Model For Fetal Alcohol Syndrome Disorders: Role For The Insulin Pathway, Rachael L. French, K D. Mcclure, U Heberlein

Rachael L. French

Prenatal exposure to ethanol in humans results in a wide range of developmental abnormalities, including growth deficiency, developmental delay, reduced brain size, permanent neurobehavioral abnormalities and fetal death. Here we describe the use of Drosophila melanogaster as a model for exploring the effects of ethanol exposure on development and behavior. We show that developmental ethanol exposure causes reduced viability, developmental delay and reduced adult body size. We find that flies reared on ethanol-containing food have smaller brains and imaginal discs, which is due to reduced cell division rather than increased apoptosis. Additionally, we show that, as in mammals, flies reared …

New Phylogenetic Information Suggests Both An Increase And At Least One Loss Of Cooperative Breeding During The Evolutionary History Of Aphelocoma Jays, Elena Berg, Robert Aldredge, A. Peterson, John Mccormack

John E. McCormack

Efforts to identify ecological and life history factors associated with cooperative breeding have been largely unsuccessful, and interest is growing in the role of phylogenetic history in determining the distribution of this social system among lineages. In birds, cooperative breeding is distributed non-randomly among lineages, suggesting that phylogenetic inertia may play an important role in determining its distribution. The bird genus Aphelocoma has been particularly well studied because, although it is a relatively small genus, it shows broad among-lineage variation in level of cooperation. Previous analyses described an unusual unidirectional pattern of evolutionary loss of cooperation in Aphelocoma. Here, historical …