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Myocyte Swelling And Plasmalemmal Integrity During Early Experimental Myocardial Ischemia In Vivo, Martin D. Sage, Robert B. Jennings
Myocyte Swelling And Plasmalemmal Integrity During Early Experimental Myocardial Ischemia In Vivo, Martin D. Sage, Robert B. Jennings
Scanning Microscopy
Using scanning and transmission electron microscopy, the structure of myocytes early in the phase of irreversible injury induced by 40 minutes of severe regional ischemia has been investigated, paying particular attention to the effects of cell swelling on the SEM appearance of the myocytes. Swollen myocytes showed an increased space beneath the plasmalemma and between organelles. True subsarcolemmal blebs were not seen and the attachment complexes between the Z-band and the underlying myofibrils remained intact. The proportion of the PS face of the plasmalemma which appeared "en face" (0.70%, SD:1.22 vs 5.0196, SD:3.72) in freeze-fracture faces of ischemic tissue was …
An Overview Of Platelet Structural Physiology, J. G. White
An Overview Of Platelet Structural Physiology, J. G. White
Scanning Microscopy
Marion Barnhart and her colleagues used light, phase contrast and scanning electron microscopy to provide a clear picture of platelet surface changes developing in response to aggregating agents. This review, in honor of Marion and her work, has sought to expand the horizon provided through study of surface alterations by peeling back the membrane of the platelet to reveal the dynamic world within. A cytoskeleton consisting of a circumferential microtubule and submembrane actin filaments supports the discoid shape of the resting cell. Following exposure to aggregating agents in suspension, to foreign surfaces or denuded blood vessels and to fibrin strands …
The Role Of The Cytoskeleton In Endothelial Repair, Avrum I. Gotlieb, Michael K. K. Wong, Patricia Boden, Alan Choo Fone
The Role Of The Cytoskeleton In Endothelial Repair, Avrum I. Gotlieb, Michael K. K. Wong, Patricia Boden, Alan Choo Fone
Scanning Microscopy
The injured endothelium undergoes rapid repair of areas of cell desquamation in order to maintain the structural integrity of the endothelial surface. Endothelial repair involves a series of processes which include endothelial cell spreading, translocation, and proliferation. These processes are well defined events which occur sequentially in time. Spreading and translocation are mediated by the cell cytoskeleton - F-actin microfilaments and microtubules and associated centrosomes. The regulation of these processes is complex and is likely due to soluble factors present at the site of injury which are released from activated endothelial cells, platelets, the subendothelial substratum, activated serum factors, and …
Megakaryocyte Motility And Platelet Formation, Robert M. Leven
Megakaryocyte Motility And Platelet Formation, Robert M. Leven
Scanning Microscopy
The mechanism of platelet formation is reviewed with special emphasis on the role of the cytoskeleton. The three major theories for platelet formation are by cytoplasmic budding, cytoplasmic dissolution or pseudopod formation. Most evidence indicates that platelets form as fragments of megakaryocyte pseudopodia. Pseudopodia formation is stimulated in vitro by thrombocytopenic rabbit plasma. It is inhibited by vincristine and altered by taxol. Cytochalasins cause pseudopodia to form in isolated megakaryocytes. Therefore, normal pseudopodia formation may depend on a combination of microfilament disorganization and microtubule elongation.
Myogenesis In Vitro As Seen With The Scanning Electron Microscope, Y. Shimada, M. Komiyama, M. Shiozaki, Y. Isobe, S. Masuko
Myogenesis In Vitro As Seen With The Scanning Electron Microscope, Y. Shimada, M. Komiyama, M. Shiozaki, Y. Isobe, S. Masuko
Scanning Microscopy
In this paper, we review our recent observations by scanning electron microscopy (SEM) on the differentiation of the cell surface and cytoplasmic organelles in embryonic chick skeletal muscle cells in vitro. The changes of the surface structures of myoblasts during mitosis were essentially similar to those of other cell types, but the characteristic spindle shape of myoblasts did not change throughout most of this period. Cytoskeletal structures under the sarcolemma were examined by Triton extraction and metal coating. Cells in S, G2 and M possessed a dense, and those in G1 a loose filament network under the …
Trifluoperazine Inhibition Of Fibrinogen Receptor Redistribution In Surface Activated Platelets: Correlative Video-Enhanced Differential Interference Contrast Light Microscopic, High Voltage Electron Microscopic And Scanning Electron Microscopic Studies, O. E. Olorundare, S. L. Goodman, R. M. Albrecht
Trifluoperazine Inhibition Of Fibrinogen Receptor Redistribution In Surface Activated Platelets: Correlative Video-Enhanced Differential Interference Contrast Light Microscopic, High Voltage Electron Microscopic And Scanning Electron Microscopic Studies, O. E. Olorundare, S. L. Goodman, R. M. Albrecht
Scanning Microscopy
Video-enhanced differential interference contrast (VDIC) light microscopy in conjunction with fibrinogen labelled colloidal gold was employed as a probe to follow the mobility of the fibrinogen receptor on platelets. Correlative studies by both high voltage and scanning electron microscopy confirms localization of labels relative to platelet ultrastructural and surface characteristics, respectively. Treatment of platelets with trifluoperazine prior to and after incubation with fibrinogen-gold labels results in a concentration dependent inhibition of receptor movement. The results obtained from this study suggest that phosphorylation of myosin by the Ca++ -calmodulin dependent enzyme, myosin-light chain kinase, is important in the fibrinogen redistribution …