Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Entire DC Network
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Undergraduate Honors Capstone Projects
The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …