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Somatic Embryogenesis From Immature Cotyledons Of Apomictic And Non-Apomictic Seeds In Walnut (Juglans Regia L.), Beki̇r Şan, Hati̇ce Dumanoğlu
Somatic Embryogenesis From Immature Cotyledons Of Apomictic And Non-Apomictic Seeds In Walnut (Juglans Regia L.), Beki̇r Şan, Hati̇ce Dumanoğlu
Turkish Journal of Agriculture and Forestry
Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen of the apple cv. 'Golden Delicious' (Malus x domestica Borkh.). The best cotyledon stage for somatic embryogenesis was determined in open-pollinated seeds of 10 walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after anthesis. As a result of this experiment, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after anthesis. Driver and Kuniyuki walnut (DKW) medium supplemented with 1 …