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Life Sciences

Theses and Dissertations

Theses/Dissertations

2016

Health and environmental sciences

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Calculating Evapotranspiration Of Arundo Donax Along The Rio Grande, Jose R. Escamilla Jr. Dec 2016

Calculating Evapotranspiration Of Arundo Donax Along The Rio Grande, Jose R. Escamilla Jr.

Theses and Dissertations

Invasive species have been recorded to cause large economic and ecological impacts on various ecosystems and their services. Locally, giant reed (Arundo donax L.), a woody grass native to the Mediterranean, adversely impacts riparian ecosystems in southwestern United States by aggressively displacing their native flora and fauna. Giant reed also has become a cause of concern for national water security, especially in water-limited areas of the arid southwestern United States. The main objective of this study is to provide the first, landscape-level estimates of water use by giant reed in the United States. Evapotranspiration was monitored using the eddy …


Developing A Multimetric Index To Assess Resaca Ecosystem Health, Buford J. Lessley Dec 2016

Developing A Multimetric Index To Assess Resaca Ecosystem Health, Buford J. Lessley

Theses and Dissertations

As the only freshwater ecosystem in the lower Rio Grande Valley aside from the Rio itself, resacas are critical habitat for many species of flora and fauna. Old distributaries of the Rio Grande, resacas provided conveyance routes moving floodwater to the Laguna Madre. Today these wetlands are novel ecosystems and are artificially maintained. Urbanization and agriculture have lead to sedimentation, habitat loss, contaminants, poor water quality, and invasive species. The objective of this study was to assess and monitor resaca pools and to compose the Resaca Health Index (RHI) from selected indicators of ecosystem structure and function including leaf litter …


The Effect Of Manganese (Mn) On Cell Proliferation And Migration In The Michigan Cancer Foundation-7 (Mcf-7) Human Breast Cancer Cell Line In Vitro, Justin Lloyd Wendel Aug 2016

The Effect Of Manganese (Mn) On Cell Proliferation And Migration In The Michigan Cancer Foundation-7 (Mcf-7) Human Breast Cancer Cell Line In Vitro, Justin Lloyd Wendel

Theses and Dissertations

Manganese (Mn) is a common environmental element necessary for mammalian physiological processes. However, dietary excess or deficiency can lead to detrimental health effects. Therefore, I investigated the effect Mn has on breast cancer progression in vitro. Utilizing MCF-7 breast cancer cells an MTT assay was performed to identify proliferative potential. After 5 days, results showed 100 nM manganese chloride (MnCl2) had a significant (p<0.01) increase in cell number over control. Using a scratch wound-healing assay, 100 nM MnCl2 was found to increase cellular motility as well as induce a possible change in morphology and cytoskeletal organization. Given Mn was shown to localize in the mitochondrial, we investigated Mn-induced ROS expression. 500 nM Mn significantly decreased (p<0.05) ROS levels. Collectively, I provide evidence that Mn may potentially drive less aggressive cancer cells towards a more invasive phenotype.


South Padre Island Beach Management: Assessment Of Dune Restoration, Shelby Rose Bessette Aug 2016

South Padre Island Beach Management: Assessment Of Dune Restoration, Shelby Rose Bessette

Theses and Dissertations

Dune restoration is a common method of increasing coastal resilience. A dune restoration program was initiated by the City of South Padre Island, TX in 2010 consisting primarily of plantings of Sea Oats, Uniola paniculata, and Bitter Panicum, Panicum amarum and has continued approximately annually thereafter. These restoration efforts were evaluated in this study by comparing dune ecological structure and function among reference sites and restoration plots ranging in age from 2 to 5 years. Plant and animal communities were examined using a combination of quadrat sampling, funnel traps, pit-falls and sweep nets. Soil organic content and soil electric …


Apoptotic And Necrotizing Mechanisms Of Recombinant Panton-Valentine Leukocidin In Thp1 Monocytes., Heriberto Deleon Iii May 2016

Apoptotic And Necrotizing Mechanisms Of Recombinant Panton-Valentine Leukocidin In Thp1 Monocytes., Heriberto Deleon Iii

Theses and Dissertations

The Panton-Valentine Leukocidin (PVL) is a highly specialized bacterial exotoxin produced by Staphylococcus aureus. The increased degree of invasiveness of community associated methicillin resistant Staphylococcus aureus (CAMRSA) has been attributed to the relatively high rates of PVL produced by the pathogen, thus increasing pathogenicity and virulence. The increased rates of PVL encoding CAMRSA infections have been correlated with increased rates of necrotizing fasciitis, invasive osteomyelitis and invasive necrotizing pneumococcal infections. Previous research as revealed that PVL has the ability to induce apoptosis at low concentration (5nM-10nM) and necrosis (50-100nM) at high concentrations, with little detail on the mechanism of initiation. …


Jatropha Curcas Protoplast Isolation And Inhibitory Effects Of Culture, Christopher S. Lukaszewski May 2016

Jatropha Curcas Protoplast Isolation And Inhibitory Effects Of Culture, Christopher S. Lukaszewski

Theses and Dissertations

Improvement on the sub-tropical plant Jatropha curcas may increase the use of this drought tolerant oilseed crop for the production of biodiesel. One of the methods for enhancing a species is through genetic manipulation of the protoplast. Isolating protoplasts is challenging and culturing has many obstacles similar to those seen in tissue culture, such as oxidative browning. Successful isolation of J. curcas leaf protoplasts resulted in an average of 3.23 x 106 viable protoplasts/mL. However, protoplasts diminished within 2 days after culturing. A review of the potential solutions used for overcoming the inhibitory effects of culture may reveal further insight …


The Acute Effects Of Aerobic Exercise With Blood Flow Restriction Cuffs On Arterial Compliance In Males And Females, Maria M. Gonzalez May 2016

The Acute Effects Of Aerobic Exercise With Blood Flow Restriction Cuffs On Arterial Compliance In Males And Females, Maria M. Gonzalez

Theses and Dissertations

RESULTS: Significant condition*gender interactions found in in SBP (p<.05), PR (p<.01), SVR (p<.01), SpO2 (p<.05), PWV carotid to femoral (p<.03), and PWV femoral to distal (p<.02). Significant condition*time interactions were found in SBP (p<.01), DBP (p<.01), MAP (p<.01), SV (p<.01), CO (p<.01), SVR (p<.01), TVI (p<.01), HR (p<.01), and SAE (p<.01). Significant condition main effects were found in SBP (p<.02), DBP (p<.03), PR (p<.03), SV (p<.01), CO (p=.01), SVR (p<.01), TVI (p<.01), HR (p<.01), and SAE (p<.03) as well as a condition trend in PWV femoral to distal (p=.06). Significant time main effects were found in SBP (p<.01), MAP (p<.04), PP (p<.01), PR (p<.01), CO (p<.01), SVR (p<.01), TVI (p<.01), HR (p<.01), SpO2 (p<.01), LAE (p<.03), and PWV femoral to distal (p<.01).

CONCLUSION: The 60 min session at a moderate aerobic intensity might have the potential to improve arterial elasticity more than a 20 min BFR session.