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Cis-Acting Signals For Replication Of Nodamura Virus Rna1, John Joseph Rosskopf
Cis-Acting Signals For Replication Of Nodamura Virus Rna1, John Joseph Rosskopf
Open Access Theses & Dissertations
The family Nodaviridae is comprised of two genera: the alphanodaviruses that infect insects and the betanodaviruses, which have been isolated only from fish. Nodamura virus (NoV), the type species of the alphanodavirus genus, can lethally infect both insects (mosquitoes, honey bees, and wax moth larvae) and mammals (suckling mice and suckling hamsters). In addition, nodavirus RNAs can replicate in a wide variety of host cells, including those of mammalian, insect, plant, and yeast origin, when introduced by transfection. The nodavirus positive strand RNA genome is naturally segregated into two segments. RNA1 encodes the viral RNA-dependent RNA polymerase (RdRp) that replicates …
The Role Of Rna Secondary Structure In Replication Of Nodamura Virus Rna2, John Howard Upton
The Role Of Rna Secondary Structure In Replication Of Nodamura Virus Rna2, John Howard Upton
Open Access Theses & Dissertations
Our laboratory studies genome replication of positive-strand RNA viruses, using a reverse-genetic system in yeast cells. Nodamura virus (NoV) provides an excellent model system for the study of RNA replication due to its genetic simplicity, its robust yield of replication products, and its ability to replicate in a wide variety of host cells. NoV contains a bipartite positive strand RNA genome: RNA1 encodes the viral RNA replicase, while RNA2 encodes the capsid protein. The role of RNA secondary structure in the genome replication of other RNA viruses has been well established. For NoV, sequences at the 3' end of RNA2 …