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Transcriptome Profiling And Long Non-Coding Rna Identification In Grapevine, Zachary Noel Harris

MSU Graduate Theses

Next-generation sequencing technologies have provided access to vast quantities of nucleic acid sequence data. The resulting wealth of information enables biologists to address complex biological questions in species for which a high-quality well-annotated reference genome sequence has yet to be generated. The cultivated grapevine, Vitis vinifera, has a relatively poorly annotated reference genome. In addition, it is a highly heterozygous species which further hinders the annotation of its genome and the characterization of its transcriptome. Here, I annotated Version 2 of the 12X V. vinifera genome using RNA-seq data derived from the variety ‘Riesling' by employing the most up-to-date computational …