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Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
LSU Master's Theses
Numerous studies have reported aberrant gene expression levels attributed to suboptimal in vitro culture conditions presented to embryos. Since the culture environment is a common aspect of both in vitro production (IVP) and nuclear transfer (NT), research focusing on the in vitro culture system will have the potential to improve both techniques. This study investigated the effects of different culture systems and protein sources on the developmental competence of IVP embryos measured by cleavage and blastocyst rates, cell number, and relative abundance of oct-4, nanog, connexin 43, and GLUT-1 transcripts when compared to in vivo embryos. Experiment 1 compared IVP …