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Follicle Stimulating Hormone Receptor Resides In Detergent Resistant Membrane Microdomains, Johanna Geoghegan
Follicle Stimulating Hormone Receptor Resides In Detergent Resistant Membrane Microdomains, Johanna Geoghegan
Honors Theses
Follicle stimulating hormone (FSH) is an anterior pituitary glycoprotein hormone crucial for proper spermatogenesis in males and oocyte development in females. The follicle stimulating hormone receptor (FSHR) is a G-protein coupled receptor found exclusively on the membranes of Sertoli cells in the testes and granulosa cells in the ovaries. It has been proposed that FSHR localizes to detergent resistant portions of the membranes called lipid rafts, which are membrane microdomains that contain a high percent composition of cholesterol and sphingolipids. To determine if FSHR resides in these membrane microdomains, strong detergents were used to extract and isolate detergent resistant membranes. …
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Honors Theses
The overall goal of this project was to investigate the biological role of a putative metacaspase gene present in the mushroom fungus Schizophyllum commune. For this study, we have utilized a strain of S. commune that is unable to integrate DNA via the non-homologous end joining pathway. This forces transforming DNA to integrate homologously, as is required for the purposes of gene knockout. The gene Scp1 encodes a likely member of the metacaspase protein family, which are suspected to have activity similar to caspases, the latter crucial to programmed cell death. A knockout construct containing a non-functional version of Scp1 …
From Gene To Behavior: Investigation Of Ppt1 And Ppt2 Rnai Induced Knock-Down During Drosophila Neurogenesis, Patrick J. O'Hern
From Gene To Behavior: Investigation Of Ppt1 And Ppt2 Rnai Induced Knock-Down During Drosophila Neurogenesis, Patrick J. O'Hern
Honors Theses
Infantile Neuronal Ceroid Lipofuscinosis (INCL) is caused by a mutation of the gene CLN1, which encodes palmitoyl protein thioesterase 1 (PPT1). PPT1 is a lysosomal enzyme that cleaves the thioester bond connecting long chain fatty acids of palmitoylated proteins. Without PPT1, palmitoylated proteins are not degraded properly and this causes unwanted build up in neurons leading to fatality. Recently, it has been thought that a mutation in a similar enzyme, palmitoyl-protein thioesterase 2 (PPT2), another lysosomal hydrolase, may also be responsible for INCL by causing a similar effect of Ppt1 deficiency. A bioinformatics analysis in this study showed similarities between …
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Honors Theses
In the fields of protein science and medicine, understanding the kinetics of protein aggregation are significant in the research and treatment of certain amyloid diseases such as Alzheimer’s. Previous studies have suggested that arginine can increase the solubility of certain proteins, suppress protein aggregation, and assist in the refolding of aggregated proteins; however, the molecular mechanisms by which arginine can influence protein aggregation are still unclear. Bovine insulin was employed as a model system for further understanding the effects of arginine on protein aggregation. Using Dynamic Light Scattering (DLS), we studied the concentration-dependent and temperature-dependent suppression of aggregation in insulin …