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Social Media Impacts On Arkansas Consumer Perceptions Of Gmos, Elizabeth R. Berner
Social Media Impacts On Arkansas Consumer Perceptions Of Gmos, Elizabeth R. Berner
Graduate Theses and Dissertations
This study builds upon previous consumer perceptions of GMOs studies. A need existed to determine what, if any, effects social media messages about GMOs had on consumer perceptions in Arkansas. The study utilized a qualitative approach with three focus groups. Data were analyzed using descriptive code. Participants were found to have a general understanding of GMOs, though discussion about what a GMO product was took an uncertain tone. Most participants did not consider GMOs as a factor when purchasing products. Rather participants considered characteristics such as price, quality, and convenience. Participants did not believe the infographics had changed their opinions …
Identification Of Possible Vectors For Histomoniasis In Turkeys On Commercial Farms In Arkansas And Oklahoma, Treva Dillon
Identification Of Possible Vectors For Histomoniasis In Turkeys On Commercial Farms In Arkansas And Oklahoma, Treva Dillon
Graduate Theses and Dissertations
Histomonas meleagridis, is a parasitic protozoan, commonly found in the gallinaceous species of birds and is the causative agent of Blackhead disease. The cecal worm, known as Heteraskis gallinarum, acts as a vector in the spread of H. meleagridis in gallinaceous birds. H. meleagridis develops inside the cecal worm and in its eggs which allows H. meleagridis a method to survive in the environment. Blackhead disease causes high mortality rates in both chickens and turkeys; however, turkeys are noted to have a higher mortality rate compared to chickens. Molecular diagnostic testing such as PCR has been developed by creating primers …
Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea
Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea
Graduate Theses and Dissertations
In Mid-South rice (Oryza sativa, L.) production, nitrogen (N) fertilizer management for pureline varieties is most often recommended as a single preflood (SPF) or two-way split (2WS) application in a direct-seeded, delayed-flood system. Most of the N fertilizer is typically applied at the four- to six-leaf stage onto dry soil, and the second application, if necessary, into the floodwater during early reproductive growth stages (referred to as midseason). Environmental factors frequently prohibit growers from applying early N fertilizer under optimal dry soil conditions. A study was conducted to determine the best N fertilization management practices to utilize in rice when …
Evaluation Of Benzobicyclon In Midsouth Rice (Oryza Sativa) Production Systems, Jake Patterson
Evaluation Of Benzobicyclon In Midsouth Rice (Oryza Sativa) Production Systems, Jake Patterson
Graduate Theses and Dissertations
Controlling weedy rice postemergence is challenging for rice producers in the United States because of the lack of herbicide options. Weedy rice is genetically similar to cultivated rice, thus making it difficult to control with mid-season postemergence herbicide applications without also damaging the crop. Hence, there is a need for a new effective postemergence weedy rice control herbicide. Findings from this research indicate that the use of benzobicyclon in current standard quizalofop- and imidazolinone-resistant rice herbicide programs provides tremendous utility for Midsouth rice producers. In both of these production systems, the addition of benzobicyclon to the respective standard herbicide programs …
The Effects Of Commercial Harvest On The Density And Demography Of Aquatic Turtles In Arkansas, Andrhea Darleen Massey
The Effects Of Commercial Harvest On The Density And Demography Of Aquatic Turtles In Arkansas, Andrhea Darleen Massey
Graduate Theses and Dissertations
The United States is home to the second highest concentration of turtle species in the world, after Asia. As of 2018, there are 57 turtle species recognized within the US, 40% of which are listed as threatened or endangered, with the primary threats to population persistence identified as over-consumption and/or habitat loss. Within the US, the Mississippi Alluvial Valley (MAV) region represents the second highest turtle species richness, after the Mobile River Basin. The MAV region of Arkansas is one of the least regulated in terms of commercial aquatic turtle harvest and has undergone large-scale habitat conversion from bottomland hardwood …
Palmer Amaranth [Amaranthus Palmeri (S.) Wats.] Resistance To S-Metolachlor In The Mid-Southern Us And S-Metolachlor Dissipation In Soil, Koffi Badou Jeremie Kouame
Palmer Amaranth [Amaranthus Palmeri (S.) Wats.] Resistance To S-Metolachlor In The Mid-Southern Us And S-Metolachlor Dissipation In Soil, Koffi Badou Jeremie Kouame
Graduate Theses and Dissertations
Palmer amaranth [Amaranthus palmeri (S.) Wats.] presents both a high genetic diversity and propensity to evolve resistance to herbicides of several sites-of-action which have made it one of the worst weeds in US agriculture. In Arkansas, Palmer amaranth is resistant to herbicides of seven sites-of-action, which are 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor, acetolactate synthase inhibitors, microtubule inhibitors, protoporphyrinogen oxidase inhibitors, very long chain fatty acid inhibitors, glutamine synthetase inhibitors, and hydroxyphenylpyruvate dioxygenase inhibitors. Sustainable management requires a better understanding of its biology and that of herbicide environmental fate. This research had five objectives: 1) characterize the current status of Palmer amaranth …
Population Connectivity Of The Eastern Collared Lizard Crotaphytus Collaris In Arkansas, Whitney Allison Murchison-Kastner
Population Connectivity Of The Eastern Collared Lizard Crotaphytus Collaris In Arkansas, Whitney Allison Murchison-Kastner
Graduate Theses and Dissertations
Habitat reduction and fragmentation can isolate populations and decrease genetic diversity, making them susceptible to local extirpation. Additionally, geographic barriers can further impede dispersal among populations thus reducing gene flow. Field studies suggest these factors may be responsible for the decline in Eastern Collared Lizard (Crotaphytus collaris) populations in Arkansas. To address the impacts of habitat loss and fragmentation on the Eastern Collared Lizard (C. collaris) in Arkansas, I used DNA fragment analysis to examine genetic diversity, population structure and connectivity among C. collaris populations. I do so herein by employing microsatellite data from 138 adults across 11 loci to …
Description Of Job Embeddedness In Arkansas County Extension Agents, Anika Parks
Description Of Job Embeddedness In Arkansas County Extension Agents, Anika Parks
Graduate Theses and Dissertations
The Cooperative Extension System (CES) has had a long-standing problem with the retention of its county Extension agents (CEAs), who are in charge of running various programs in over 3,000 counties within the United States. When a CEA leaves a county, voluntarily or involuntarily, the community members are left without that leader, and their needs go unmet, which was proposed to increase voluntary turnover among CEAs even further. Arkansas CES has had its problems with turnover, and studying this issue through the lens of Job Embeddedness Theory was identified as a gap in the literature.
Job Embeddedness Theory is a …
An Evaluation Of Biopesticide Combinations On Yield Performance And Disease/Arthropod Control Of Strawberries Grown In High Tunnel Plasticulture Production Systems In Arkansas., Karlee B. Pruitt
Graduate Theses and Dissertations
This two-year study investigated combinations of biopesticides to determine impacts on strawberry fruit marketable fruit yields, and effectiveness in controlling strawberry pests in a high tunnel production system at the University of Arkansas, Division of Agriculture Research and Extension Center in Fayetteville, Arkansas. Two strawberry cultivars Fragaria × ananassa (Duch.), Camino Real and Sweet Sensation were grown in a high tunnel from early-October to mid-May for two consecutive growing seasons, (2017-18 and 2018-19) with six treatment combinations of biopesticides including an untreated (water) control, nutrient spray and selected biological based fungicides and insecticides, arranged into a split-plot randomized block design. …
Imperfect Produce Pricing: Relationships Between Price Percent Discount And Demographic Traits Of Customers, April Foster
Imperfect Produce Pricing: Relationships Between Price Percent Discount And Demographic Traits Of Customers, April Foster
Graduate Theses and Dissertations
This research sought to understand the impact of price reductions on consumer spending behaviors related to the purchase of imperfect produce. Additionally, the correlation between consumers’ demographics and their willingness to purchase imperfect produce at various price points was evaluated. The data was viewed with mindfulness toward reducing food loss as a function of the world food crisis. More specifically, the purpose of this study was to describe the relationship between Washington County, Arkansas consumers’ demographic traits and the percent discount at which they are willing to alter their behavior to purchase specific imperfect produce items. Using the Theory of …
Where Are All Of Arkansas' Chinquapins? An Ecological Assessment Of Castanea Throughout The State, Logan Pierce Estes
Where Are All Of Arkansas' Chinquapins? An Ecological Assessment Of Castanea Throughout The State, Logan Pierce Estes
Graduate Theses and Dissertations
Around the turn of the twentieth-century, the chestnut blight fungus (Cryphonectria parasitica) was accidentally introduced into North America. This strong pathogen, which specializes on trees of the genus Castanea, spread rapidly and within half a century had nearly extirpated North America’s Castanea natives from their ranges. During this catastrophe, the American chestnut (Castanea dentata) garnered much of the scientific attention, pushing the other Castanea natives – the chinquapins – to the wayside. More than a century following the spread of the blight, little research into the ecology of North America’s chinquapins had been performed, leaving these trees significantly underrepresented. The …
Risk And Return Comparisons Of Pre-Harvest Marketing Strategies, John Leander Turner V
Risk And Return Comparisons Of Pre-Harvest Marketing Strategies, John Leander Turner V
Graduate Theses and Dissertations
This paper analyzes risk and returns associated with pre-harvest corn grain marketing strategies for the state of Arkansas. Farming is characterized by a volatile environment. Numerous risks are taken by producers in order to provide commodities that are bought and sold by various parties in the supply chain. Price, yield, and production costs vary daily and can have large variation between years. Risk and Return Comparisons of Pre-harvest Marketing Strategies examines the effectiveness of using pre-harvest marketing strategies to enhance returns and to mitigate inherent price risk in the Memphis cash corn market. Thirteen strategies are compared to the October …
Aiding Growers’ Decisions: Describing Arkansas Blackberry Growers’ Resources And Needs Regarding Blackberry Production, Lesley Marie Smith
Aiding Growers’ Decisions: Describing Arkansas Blackberry Growers’ Resources And Needs Regarding Blackberry Production, Lesley Marie Smith
Graduate Theses and Dissertations
This study was a census of blackberry growers who attended the Blackberry Growers Association informal interest meetings held throughout the state during the month of April. The purpose of this study was to describe blackberry operations in Arkansas, describe the ideal blackberry cultivar growers want to produce, describe resources they use to research production information, and describe what changes to current resources growers would like to have. Questionnaire responses regarding blackberry production of 18 blackberry growers in the state of Arkansas were analyzed. Three growers elected to not participate resulting in an 86% response rate. The researcher hopes that this …
Nitrous Oxide Emissions From Rice Production On A Silt-Loam Soil In Arkansas, Casey Rector
Nitrous Oxide Emissions From Rice Production On A Silt-Loam Soil In Arkansas, Casey Rector
Graduate Theses and Dissertations
Rice (Oryza sativa L.) is a common crop grown in Arkansas under flooded-soil conditions. The saturated to nearly saturated soil makes rice production an ideal environment for the production of potent greenhouse gases, such as nitrous oxide (N2O). The objectives of this study were to i) evaluate the impact of water management practice (full-season-flood and intermittent-flood) and cultivar (pure-line and hybrid) on N2O fluxes, season-long N2O emissions, and global warming potential (GWP; 2016) and ii) evaluate the impact of tillage practice [conventional tillage and no-tillage (NT)] and type of urea fertilizer [ N-(n-butyl) thiosphosphoric triamide (NBPT)-coated and non-coated urea] on …
A Study Of The Prevalence Of Gastrointestinal Nematodes In Goats Obtained From Northwest Arkansas, Christine Rose Weingartz
A Study Of The Prevalence Of Gastrointestinal Nematodes In Goats Obtained From Northwest Arkansas, Christine Rose Weingartz
Graduate Theses and Dissertations
Parasitic helminths have always been an issue in small ruminant production; pathogens that pose great negative impact on goat health and productivity. Insufficient work has been done to document the prevalence of parasitic helminths in the United States, especially in the south and southeast, where the largest goat populations are found.
The aim of this study was to survey the prevalence of infections by gastrointestinal nematodes in goats in Northwestern Arkansas. Gastrointestinal tracts were examined from 41 goats of various locations around Northwest Arkansas. Worm species were identified and population burdens were determined. Coprology was correlated with the actual worm …
Evaluation Of Insecticide Seed Treatments To Protect Rice (Oryza Sativa) Against Various Herbicides, Steven Michael Martin
Evaluation Of Insecticide Seed Treatments To Protect Rice (Oryza Sativa) Against Various Herbicides, Steven Michael Martin
Graduate Theses and Dissertations
The increase in herbicide-resistant weeds in Arkansas crop fields has led to the need for new herbicide modes of action for use in all crops. This need has led to the introduction of technologies that can be devastating to conventional rice crops. Field observation, noted that insecticide seed treatments used in rice could potentially reduce the effects of off-target movement of herbicides onto rice crops and possibly reduce the negative effects of some herbicides applied directly to rice. Research was conducted to determine if insecticide seed treatments could reduce the harmful effects of drift from imazethapyr and glyphosate onto conventional …
Evaluating Methods For Quantifying Evapotranspiration In Arkansas Rice, Colby Reavis
Evaluating Methods For Quantifying Evapotranspiration In Arkansas Rice, Colby Reavis
Graduate Theses and Dissertations
The goal of this study was to evaluate different methods for quantifying evapotranspiration (ET) in commercial rice fields using different irrigation regimes. The rice fields were located in south central Arkansas. The different irrigation regimes were alternate wetting and drying (AWD) and continuous flooding (CF). Alternate wetting and drying and conventional flooding estimates of ET were 602 mm and 570 mm, respectively, based on field observations using eddy covariance. Models used to estimate ET estimated values between 498 and 653 mm for the 2015 growing season. The Penman Monteith actual evapotranspiration model (PM AET) performed best when compared to the …
Environmental And Climatic Constraints On Large-Scale Camelina Production In Eastern Arkansas, Benjamin Robert Tracy
Environmental And Climatic Constraints On Large-Scale Camelina Production In Eastern Arkansas, Benjamin Robert Tracy
Graduate Theses and Dissertations
Camelina sativa is a cold weather crop that is typically grown in semi-arid environments in the Western United States, usually as a spring crop, but sometimes during the winter. Research analyzing climate data and soil hydrology is important to better understand the environmental and terrain conditions necessary for Camelina farming wherever it is proposed for large-scale production. This study focused on various conditions and constraints pertaining to the potential for Camelina as a crop biofuel in Eastern Arkansas. Due to interest in the economic potential of crop biofuels in this area, and in particular the low input costs for Camelina, …
Cover Crop Establishment And Potential Benefits To Arkansas Farmers, Ashley Elizabeth Humphreys
Cover Crop Establishment And Potential Benefits To Arkansas Farmers, Ashley Elizabeth Humphreys
Graduate Theses and Dissertations
Soybean farmers in Arkansas need best management practices (BMPs) that maximize the benefits of using cover crops including planting date and fertilization recommendations. An evaluation of cover crop species, planting dates, seeding rates, fertilizer rates, and N accumulation aids in providing these BMPs. The first objective of this research is to assess the effect of planting date on biomass production, as well as looking at the interaction of seeding rate or fertilizer rate for legumes or non-legumes, respectively, using Austrian winter pea (Pisum sativum), cereal rye (Secale cereale), black oats (Avena strigosa), wheat (Triticum aestivum), and tillage radish (Raphanus sativus). …
Microbes In Rough Rice – Prevalence, Growth Kinetics And Links To Dry Matter Loss, Supriya Thote
Microbes In Rough Rice – Prevalence, Growth Kinetics And Links To Dry Matter Loss, Supriya Thote
Graduate Theses and Dissertations
The prevalence of microorganisms on freshly-harvested long-grain hybrid, long-grain pureline, and medium-grain rice has not been elucidated. The first objective of this two-part study was to determine the relative prevalence of microbes on freshly-harvested, high moisture content rice of different types and cultivars. The total and surface aerobic plate counts (APC) and mold counts were determined in terms of log10 colony forming units per gram of rice (log10 CFU/g) for six rice cultivars over two years. In 2013, among harvested samples of all the studied rice cultivars, the long-grain hybrid cultivars had the lowest APC levels (M = 8.10, SD …
Comparisons Of Selected Household And Commercial Disinfectants Against Poultry Salmonella Isolates And A Survey Of Internal Parasites In Backyard Poultry In Arkansas, Kayleigh Moyle
Graduate Theses and Dissertations
Backyard and exhibition poultry have been gaining in popularity and as such there has been a large increase in the number of small flocks. As the interaction with poultry has increased, so has the opportunity for diseases and parasites, for both birds and people. One of the major zoonotic illnesses is caused by the bacteria Salmonella, which can be found in commercial and small flocks. Salmonella is the number 2 contributor of foodborne illnesses so its prevalence in commercial flocks is of high concern. Despite improved cleaning, disinfection, and biosecurity practices, there is still potential for disease outbreaks and infections …
The Effects Of Meloidogyne Incognita And Heterodera Glycines On The Yield And Quality Of Edamame In Arkansas, Juliet Fultz
The Effects Of Meloidogyne Incognita And Heterodera Glycines On The Yield And Quality Of Edamame In Arkansas, Juliet Fultz
Graduate Theses and Dissertations
Edamame (Glycine max), also known as vegetable soybean, was introduced to the United States from Japan in 1890 and has been growing in popularity as a nutrient-rich, low-sugar snack in recent years. In 2012, the American Vegetable Soybean and Edamame, Inc. established the first domestic commercial processing plant in Mulberry, Arkansas and contracted local growers for production. Since the crop is harvested when seed are immature, management practices are different from those for traditional soybean. Plant-parasitic nematodes, particularly Meloidogyne incognita (southern root-knot) and Heterodera glycines (soybean cyst), are common in Arkansas and are pests of concern for edamame growers. Edamame …
The Abundance And Distribution Of Mallards In The Lower Mississippi Alluvial Valley Of Arkansas, John Andrew Herbert
The Abundance And Distribution Of Mallards In The Lower Mississippi Alluvial Valley Of Arkansas, John Andrew Herbert
Graduate Theses and Dissertations
The management of wintering waterfowl in North America requires flexibility because of constantly changing landscapes and conditions. Many mallards use the lower Mississippi Alluvial Valley (MAV) for wintering habitat, making this an area of emphasis for improving management strategies. In this study, I used mallard observation data from 2009-2014 aerial surveys collected in the Arkansas portion of the lower MAV to explain the abundance and distribution and of mallards. Using spatial hierarchical models and breaking covariate data to 2x2 km grid cells, I analyzed how covariates relate to the changes of abundance and distributions within and among surveys. Mallard abundance …
Use Of Hppd-Inhibiting Herbicides For Control Of Common Weeds In Arkansas And Current Status Of Herbicide-Resistance Among Echinochloa Populations In Arkansas, Clay Starkey
Graduate Theses and Dissertations
Herbicide-resistant weeds in Arkansas cause problems for growers. Up-to-date information and new technologies can help plan mitigation strategies to slow resistant weeds. The objectives of this research were to provide a ‘snapshot’ of herbicide-resistant Echinochloa spp. in rice producing counties, determine how much resistance has spread across the state, and understand the effectiveness of 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides for control of glyphosate-resistant (GR) Palmer amaranth relative to commercial standards currently labeled in soybean. To assess the prevalence of Echinochloa spp. resistance, 82 samples were collected from 23 rice producing counties in 2010. The samples were tested for resistance to commonly …
Modifying A Cow-Calf Biophysical Simulation Model For Analyses Of Alternative Enterprises, Jennifer L. Lutes
Modifying A Cow-Calf Biophysical Simulation Model For Analyses Of Alternative Enterprises, Jennifer L. Lutes
Graduate Theses and Dissertations
Cow-calf producers in the United States, tasked with providing beef calves for the beef industry, have had a multitude of difficulties to overcome in recent years. Producers in northwest Arkansas were negatively impacted by high hay prices coupled with low beef cattle market prices due to severe drought experienced in portions of 2010, 2011, and 2012. During this time they also faced high grain prices, due to a record low harvest, combined with portions of the corn harvest diverted from human and animal feed to ethanol production. Tight lending policies of this time, reminiscent of the housing market crash in …
Long-Term Effects Of Alternative Residue Management Practices On Near-Surface Soil Properties And Soybean Production In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Christopher Ryan Norman
Long-Term Effects Of Alternative Residue Management Practices On Near-Surface Soil Properties And Soybean Production In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Christopher Ryan Norman
Graduate Theses and Dissertations
Adoption of management practices that maintain or increase soil organic matter (SOM), which contains 58% carbon (C) on average, may help to mitigate climate change by sequestering atmospheric C. Therefore, the main objective of this study was to determine the long-term trends in SOM, soil C and nitrogen (N), bulk density, various soil chemical properties (i.e., pH, electrical conductivity [EC], and Mehlich-3-extractable nutrients) in the top 10 cm, and soybean yield as affected by residue burning (burning and non-burning), tillage (conventional and no-tillage), irrigation (irrigated and non-irrigated), and N-fertilization/residue level (high and low) in a wheat (Triticum aestivum L.)-soybean [Glycine …
The Impact Of A Ballot Education Program On Arkansas Voters, Kristin Netterstrom Higgins
The Impact Of A Ballot Education Program On Arkansas Voters, Kristin Netterstrom Higgins
Graduate Theses and Dissertations
The University of Arkansas Division Of Agriculture’s Public Policy Center has educated Arkansas voters about statewide ballot issues for 10 years. The ballot issue education program, was evaluated during the 2014 election cycle to determine the program’s impact on voters. This descriptive study sought to describe program participants, to determine knowledge transfer of county agents, to describe knowledge acquisition of program participants, and to measure whether people who attended Cooperative Extension Service presentations made informed choices on Election Day. Researchers found that program participants were mostly older, educated, White women. There were increases in knowledge among participants who read fact …
Distribution Of The Egyptian Goose (Alopochen Aegyptiacus) In Northwestern Arkansas And In The United States Of America, Cameron Chesbro
Distribution Of The Egyptian Goose (Alopochen Aegyptiacus) In Northwestern Arkansas And In The United States Of America, Cameron Chesbro
Graduate Theses and Dissertations
The Egyptian Goose (Alopochen aegyptiacus) is an exotic member of the Anatidae that has been residing in northwestern Arkansas since the 1980s (Smith and James 2012). Following the discovery of Egyptian Geese in the area, not much attention has been given to the consequences of population increase. The Egyptian Goose has shown many diverse population growth patterns in areas where it has been introduced and started feral populations. The purpose of this study was to assess the current population size of the Egyptian Goose in northwestern Arkansas,and confirm successful breeding. The methods used in this study included conducting road surveys …
Monitoring Abundance Of Ips Bark Beetles And Determining Related Tree Mortality In Arkansas And Texas, Chandler Stefan Barton
Monitoring Abundance Of Ips Bark Beetles And Determining Related Tree Mortality In Arkansas And Texas, Chandler Stefan Barton
Graduate Theses and Dissertations
The abundance of the southern pine engraver beetles, Ips avulsus (Eichhoff), I. grandicollis (Eichhoff), and I. calligraphus (Germar), was monitored with pheromone-baited traps in 2012 and 2013 in Arkansas and eastern Texas. Additionally, vehicle-based and comprehensive ground-based surveys were used to estimate and confirm the amount of dead and fading trees in each trapping site. Pine engraver beetles have historically been recognized as secondary invaders of healthy trees, but observations of pine mortality in Texas and Arkansas appeared to correspond with high population densities of Ips spp. Forest sites exhibiting subjectively high or low incidence of Ips-associated mortality in Arkansas …
Identification Of Root-Knot Nematodes (Meloidogyne Spp.) Of Arkansas Using Molecular Diagnostics, Churamani Khanal
Identification Of Root-Knot Nematodes (Meloidogyne Spp.) Of Arkansas Using Molecular Diagnostics, Churamani Khanal
Graduate Theses and Dissertations
Root-knot nematodes (Meloidogyne spp.) are highly-adaptable, obligate plant parasites distributed worldwide. In addition, root-knot nematodes are an economically important genus of plant-parasitic nematodes. Meloidogyne incognita, M. arenaria, M. javanica, M. hapla and M. graminis have been reported from Arkansas during 1964 to 1994. Previous identifications were based primarily on morphological characters and host differentials. In this study, identification using molecular diagnostics methods was performed to identify Meloidogyne species present in Arkansas. A total of 106 soil and root samples from 36 of the 75 counties were collected and processed to obtain root-knot nematodes. Polymerase chain reaction (PCR) was performed …