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Mucosa-Associated Invariant T Cell Roles And Local Responses In Cell-Mediated Immunity To Influenza A Virus, Christine Marie Wardell
Mucosa-Associated Invariant T Cell Roles And Local Responses In Cell-Mediated Immunity To Influenza A Virus, Christine Marie Wardell
Electronic Thesis and Dissertation Repository
Mucosa-associated invariant T (MAIT) cells are innate-like T cells that are activated by microbial vitamin B metabolites and/or cytokine stimulation to produce pro-inflammatory cytokines and cytotoxic granules. Influenza A viruses (IAVs) activate MAIT cells in a by-stander manner, and their abundance negatively correlates with infection-induced morbidity and mortality. However, how MAIT cells directly contribute to anti-viral immunity is ill-defined. I hypothesized that MAIT cells’ presence in mice enhances antigen-specific CD8+ T cell responses. I observed a trend that MAIT cell-deficient mice generally developed smaller populations of antigen-specific CD8+ T cells following IAV exposure than mice abundant in MAIT …
Elucidating The Role Of Cathepsin B In The Lifecycle Of Influenza A Virus, Macon D. Coleman
Elucidating The Role Of Cathepsin B In The Lifecycle Of Influenza A Virus, Macon D. Coleman
Electronic Thesis and Dissertation Repository
Influenza virus type A (IVA) is the etiologic agent responsible for the febrile respiratory illness referred to as the flu. Seasonal and occasionally pandemic IVA-associated illness is a significant cause of morbidity and mortality worldwide, and presents a significant burden to the healthcare system. Our previous work showed that the propagation of IVA required the lysosomal protease cathepsin B (CTSB), though the mechanism behind this dependency was not elucidated. This study further examined the role of CTSB by using CTSB-deficient (CTSB-/-) macrophages and the CTSB-specific chemical inhibitor CA-074 Me (CaMe) in human lung epithelial cells. CTSB-/- and …
The Effect Of Dendritic Cell Mobilization On Cd8+ T Cell Responses To Influenza A Virus, Adil N. Shivji
The Effect Of Dendritic Cell Mobilization On Cd8+ T Cell Responses To Influenza A Virus, Adil N. Shivji
Electronic Thesis and Dissertation Repository
Influenza A viruses (IAV) cause respiratory infections with potentially catastrophic consequences. Neutralizing antibodies towards surface proteins of IAV prevent reinfection. However, mutations in these proteins allow the virus to evade these antibodies. Enhancing cytotoxic T cell-mediated immunity has been proposed as an attractive strategy to combat flu. Recombinant human FMS-like tyrosine kinase 3 ligand (FL) is known to mobilize dendritic cells (DCs) in mice. Given that DCs are the most potent antigen-presenting cells, I hypothesized that their mobilization by FL will improve the CD8+ T cell response to IAV. Quantification of CD8+ T cell responses to IAV epitopes by intracellular …