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Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan
Role Of Carotenoid Cleavage Dioxygenases In Volatile Emissions And Insect Resistance In Arabidopsis, Shailu Lakshminarayan
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In Arabidopsis thaliana, carotenoid degradation by enzymatic oxidative cleavage is catalyzed by nine CCD and NCED enzymes; this cleavage produces an array of terpenoid products that are collectively known as apocarotenoids, which include volatile and non-volatile compounds. Previous studies on CCD1 revealed that its overexpression in A. thaliana enhances β-ionone emission and reduces damage caused by flea beetles. In consideration of these findings, I generated CCD4 and CCD8 Arabidopsis overexpression lines. Further, I characterized these overexpression lines on the basis of their carotenoid content and their volatile profiles. Surprisingly, accumulation of key carotenoids in the leaves was observed in both …
Uncovering The Molecular Link Between Mir156.Spl15 And Carotenoid Accumulation In Arabidopsis, Davood Emami Meybodi
Uncovering The Molecular Link Between Mir156.Spl15 And Carotenoid Accumulation In Arabidopsis, Davood Emami Meybodi
Electronic Thesis and Dissertation Repository
Carotenoid Cleavage Dioxygenases (CCDs) are an enzyme family that cleaves specific double bonds in carotenoids. MicroR156 in Arabidopsis regulates a network of genes by repressing 10 SPL genes, among which, SPL15 was found to regulate shoot branching and carotenoid accumulation. The expression of CCD1, CCD4, CCD7, CCD8, NCED2, NCED3, NCED5, NCED6, NCED9 and SPL15 was evaluated in siliques at 10 days post anthesis and in 10-day-old roots in Arabidopsis wild type, sk156 (miR156 overexpression mutant), RS105 (miR156 overexpression line), spl15 (SPL15 knockout mutant) and two 35S:SPL15 lines. Results showed that most of CCD/NCED genes were …