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Electronic Thesis and Dissertation Repository

Theses/Dissertations

2012

Arabidopsis thaliana

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Create The Scene And Watch The Show Unfold: Following Vegetative To Embryonic Developmental Transitions By Over-Expressing Lec2 In Leaves Of Arabidopsis Thaliana, Mistianne Feeney Jun 2012

Create The Scene And Watch The Show Unfold: Following Vegetative To Embryonic Developmental Transitions By Over-Expressing Lec2 In Leaves Of Arabidopsis Thaliana, Mistianne Feeney

Electronic Thesis and Dissertation Repository

During seed development, the lytic vacuole (LV) is replaced by a protein storage vacuole (PSV) which specializes in accumulating seed storage proteins (SSPs). As seed protein reserves are mobilized upon germination, the PSV is once again replaced by the LV which takes on different roles in vegetative tissues. Cellular events occurring during these developmental transitions are not well understood, particularly, the transition between vacuole types. This research investigates whether PSVs can exist in leaves. To study vacuole transitions in leaves, an Arabidopsis thaliana line over-expressing the LEAFY COTYLEDON2 (LEC2) transcription factor was used. LEC2 is a master regulator of embryogenesis …