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A Genetic And Physiological Study Of An Arsenite Resistant, Uncoupled Mutant Of Escherichia Coli, Daniel Gordon Smiley
A Genetic And Physiological Study Of An Arsenite Resistant, Uncoupled Mutant Of Escherichia Coli, Daniel Gordon Smiley
Dissertations and Theses
Chromosomally determined arsenate resistance in Escherichia coli is well characterized. Little, however, is known about chromosomally determined arsenite resistance in E. coli. Accordingly, spontaneous arsenite resistant mutants were selected in a plasmid free strain of E. coli. One mutant strain was analyzed in detail, genetically and physiologically. The mutation confering arsenite resistance was shown to be a single gene mutation. Genetic mapping studies using conjugation and transduction showed that the mutation was closely linked to the ilv region of the E. coli map.
Plasmid-Mediated Resistance To Arsenite And Arsenate In Escherichia Coli, Thomas Patrick Trezona
Plasmid-Mediated Resistance To Arsenite And Arsenate In Escherichia Coli, Thomas Patrick Trezona
Dissertations and Theses
Bacterial strains resistant to arsenite, arsenate (or both) are readily isolated from nature. The resistance, in many cases, can be shown to be determined by conjugal plasmids. The mechanisms by which these plasmids confer resistance to arsenic compounds are entirely unknown. This study was undertaken to shed light on some of the physiological and genetic aspects of this resistance using plasmid-bearing strains of Escherichia coli which are highly resistant to both arsenite and arsenate. Most studies were carried out using E. coli J53 (R773) or the plasmid-free strain J53.