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Measurement Of Fungal Biomass In Wooden Art Objects Using Atp Photometry And Energy Charge, Fernando Nieto
Measurement Of Fungal Biomass In Wooden Art Objects Using Atp Photometry And Energy Charge, Fernando Nieto
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In this study ATP photometry and energy charge (EC) measurements were used to detect and quantify fungal biomass in wood. Three species of wood decaying fungi were used: Phanerochaete chrysosporium (white rot), Poria placenta (brown rot), and Chaetomnium globosum (soft rot). The fungi were grown in malt extract broth (MEB) for one month at 25 °C, and sampled at selected intervals to measure the ATP content and the EC of the mycelium. The mycelia were ground and extracted with cold 5% trichloroacetic acid (TCA) for 20 hours. The conversion factors calculated were based upon the EC of the mycelium. When …