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Random Amplified Polymorphic Dna (Rapd) Analysis Of Long-Term Cultured Hybrid Hazelnut, Mehmet Nuri Nas, Nedim Mutle, Paul Read

Department of Agronomy and Horticulture: Faculty Publications

RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropogated plants (6-year cultures), and no somaclonal variation was detected among in vitro propogated plants. However, polymorphism (15.6%) was detected between the parent plant and its …