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Improved Rooting And Acclimatization Of Micropropagated Hazelnut Shoots, Mehmet Nuri Nas, Paul Read

Department of Agronomy and Horticulture: Faculty Publications

Microshoots of four hazelnut genotypes grown in vitro on Nas and Read medium (NRM) containing various combinations of CuSO₄ ∙ 5H₂O and myo-inositol were successfully rooted and acclimatized ex vitro without any need of in vitro hardening treatments. Dipping of shoot bases in 1000 ppm indole-3-butyric acid (IBA) solution for 5 or 10 seconds followed by placement of shoots in plant growth regulator free NRM gave rise to formation of roots as early as 8 days. Shoots treated for 5 and 10 seconds rooted similarly, and depending on genotype, 88%-98% rooting was observed within 15 days after …