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Improved Rooting And Acclimatization Of Micropropagated Hazelnut Shoots, Mehmet Nuri Nas, Paul Read
Improved Rooting And Acclimatization Of Micropropagated Hazelnut Shoots, Mehmet Nuri Nas, Paul Read
Department of Agronomy and Horticulture: Faculty Publications
Microshoots of four hazelnut genotypes grown in vitro on Nas and Read medium (NRM) containing various combinations of CuSO4 ∙ 5H2O and myo-inositol were successfully rooted and acclimatized ex vitro without any need of in vitro hardening treatments. Dipping of shoot bases in 1000 ppm indole-3-butyric acid (IBA) solution for 5 or 10 seconds followed by placement of shoots in plant growth regulator free NRM gave rise to formation of roots as early as 8 days. Shoots treated for 5 and 10 seconds rooted similarly, and depending on genotype, 88%-98% rooting was observed within 15 days after …
Random Amplified Polymorphic Dna (Rapd) Analysis Of Long-Term Cultured Hybrid Hazelnut, Mehmet Nuri Nas, Nedim Mutle, Paul Read
Random Amplified Polymorphic Dna (Rapd) Analysis Of Long-Term Cultured Hybrid Hazelnut, Mehmet Nuri Nas, Nedim Mutle, Paul Read
Department of Agronomy and Horticulture: Faculty Publications
RAPD and phenotypic analysis were conducted to assess clonal stability of hazelnuts generated from axillary buds cultured in vitro for long-term. The nuts produced on in vitro-propagated plants were indistinguishable from those of donor plants. With the exception of rare horizontal (plagiotropic) growth, all in vitro-propagated plants exhibited phenotypes similar to those of donor plants. RAPD analysis did not reveal any somaclonal variation between donor plants from which in vitro cultures were initiated and micropropogated plants (6-year cultures), and no somaclonal variation was detected among in vitro propogated plants. However, polymorphism (15.6%) was detected between the parent plant and its …