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Department of Agronomy and Horticulture: Faculty Publications
Apigeninidin; Cochliobolus heterostrophus; Cytochrome P450; Eriodictyol; Flavonoid 30-hydroxylase; Luteolinidin; Naringenin; Phytoalexin; Sorghum bicolor; 3-Deoxyanthocyanidins
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Expression Of A Putative Flavonoid 3'-Hydroxylase In Sorghum Mesocotyls Synthesizing 3-Deoxyanthocyanidin Phytoalexins, Jayanand Boddu, Catherine Svabek, Rajandeep Sekhon, Amanda Gevens, Ralph L. Nicholson, A. Daniel Jones, Jeffrey F. Pedersen, David L. Gustine, Surinder Chopra
Expression Of A Putative Flavonoid 3'-Hydroxylase In Sorghum Mesocotyls Synthesizing 3-Deoxyanthocyanidin Phytoalexins, Jayanand Boddu, Catherine Svabek, Rajandeep Sekhon, Amanda Gevens, Ralph L. Nicholson, A. Daniel Jones, Jeffrey F. Pedersen, David L. Gustine, Surinder Chopra
Department of Agronomy and Horticulture: Faculty Publications
In sorghum, ingress of Cochliobolus heterostrophus stimulates the synthesis of 3-deoxyanthocyanidins that act as phytoalexins. Apigeninidin and luteolinidin are two major phytoalexins induced in the first 24 h after infection. In an attempt to understand genetic regulation of the biosynthesis of sorghum phytoalexins, we isolated a differentially expressed partial cDNA. Characterization and comparison showed that this cDNA sequence corresponds to a putative flavonoid 3’-hydroxylase. Full length sequence characterization allowed us to establish that the sorghum putative f3’h cDNA encodes a peptide of 517 amino acids that has domains conserved among cytochrome P450 proteins functioning in …