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Cost-Efficient And Easy To Perform Pcr-Based Assay To Identify Met Exon 14 Skipping In Formalin-Fixed Paraffin-Embedded (Ffpe) Non-Small Cell Lung Cancer (Nsclc) Samples, Odharnaith O'Brien, Mark C. Wright, Cathal O'Brien, Orla Geoghegan, Niamh Leonard, Siobhan Nicolson, Sinead Cuffe, Aurelie Fabre, Wolfram Jochum, Markus Joerger, Steven G. Gray, Stephen P. Finn
Cost-Efficient And Easy To Perform Pcr-Based Assay To Identify Met Exon 14 Skipping In Formalin-Fixed Paraffin-Embedded (Ffpe) Non-Small Cell Lung Cancer (Nsclc) Samples, Odharnaith O'Brien, Mark C. Wright, Cathal O'Brien, Orla Geoghegan, Niamh Leonard, Siobhan Nicolson, Sinead Cuffe, Aurelie Fabre, Wolfram Jochum, Markus Joerger, Steven G. Gray, Stephen P. Finn
Articles
MET is a receptor tyrosine kinase (RTK) that plays important roles in carcinogenesis. Despite being frequently overexpressed in cancer, clinical responses to targeting this receptor have been limited. Recently novel splicing mutations involving the loss of exon 14 (called METex14 skipping) have emerged as potential biomarkers to predict for responsiveness to targeted therapies with Met inhibitors in non-small cell lung cancer (NSCLC). Currently, the diverse genomic alterations responsible for METex14 skipping pose a challenge for routine clinical diagnostic testing. In this report, we examine three different methodologies to detect METex14 and assess their potential utility for use as a diagnostic …
Detection Of “Xisco” Gene For Identification Of Streptococcus Pneumoniae Isolates, Francisco Salvà-Serra, Gwendolyn Connolly, Edward Moore, Lucia Gonzales-Siles
Detection Of “Xisco” Gene For Identification Of Streptococcus Pneumoniae Isolates, Francisco Salvà-Serra, Gwendolyn Connolly, Edward Moore, Lucia Gonzales-Siles
Articles
We describe a PCR-assay differentiating Streptococcus pneumoniae from closely-related species of the Mitis group of the genus Streptococcus and identification of pneumococcus clinical isolates, based on the “Xisco” gene discriminatory marker. The complete “Xisco” gene sequence was observed in all S. pneumoniae genomes analyzed and absent in all non-pneumococcus genomes.
Isolation And Characterization Of Toxin A-Negative, Toxin B-Positive Clostridium Difficile In Dublin, Ireland, Denise Drudy, N. Harnedy, S. Fanning, R. O’Mahony, L. Kyne
Isolation And Characterization Of Toxin A-Negative, Toxin B-Positive Clostridium Difficile In Dublin, Ireland, Denise Drudy, N. Harnedy, S. Fanning, R. O’Mahony, L. Kyne
Articles
Clostridium difficile is a major cause of infectious diarrhoea in hospitalised patients. Most pathogenic C. difficile strains produce two toxins, A and B; however, clinically relevant toxin A-negative, toxin Bpositive (A– B+ ) strains of C. difficile that cause diarrhoea and colitis in humans have been isolated worldwide. The aims of this study were to isolate and characterise A– B+ strains from two university hospitals in Dublin, Ireland. Samples positive for C. difficile were identified daily by review of ELISA results and were cultured on selective media. Following culture, toxin-specific immunoassays, IMR-90 cytotoxicity assays and PCR were used to analyse …
Comparison Of Dna Extraction From Cervical Cells Collected In Preservcyt Solution For The Amplification Of Chlamydia Trachomatis, Helen Keegan, Clara Boland, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin
Comparison Of Dna Extraction From Cervical Cells Collected In Preservcyt Solution For The Amplification Of Chlamydia Trachomatis, Helen Keegan, Clara Boland, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin
Articles
Objective: The aim of this study was to compare and evaluate three methods of DNA extraction for the amplification of Chlamydia trachomatis in uterine cervical samples collected in PreservCyt solution. ThinPrep is the trade name for the slide preparation. Methods: Thirty-eight samples collected in LCx buffer medium, which were identified as C. trachomatis infected by ligase chain reaction (LCR), were selected for this study. DNA from the PreservCyt samples was extracted by three methods: (i) QIAamp kit, (ii) boiling in Tris-EDTA buffer with Chelex purification, and (iii) Proteinase K digestion with Chelex purification. Sample DNA was tested for the presence …
Real-Time Reverse Transcription Pcr For Detection And Quantitative Analysis Of Equine Influenza Virus., Michelle Quinlivan, Eugene Dempsey, Fergus Ryan, Sean Arkins, Ann Cullinnane
Real-Time Reverse Transcription Pcr For Detection And Quantitative Analysis Of Equine Influenza Virus., Michelle Quinlivan, Eugene Dempsey, Fergus Ryan, Sean Arkins, Ann Cullinnane
Articles
Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious …
Validation Of A Multiplex Pcr Assay For The Simultaneous Detection Of Human Papillomavirus And Chlamydia Trachomatis In Cervical Preservcyt Samples., Helen Keegan, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin
Validation Of A Multiplex Pcr Assay For The Simultaneous Detection Of Human Papillomavirus And Chlamydia Trachomatis In Cervical Preservcyt Samples., Helen Keegan, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin
Articles
No abstract provided.
Detection Of Five Common Cftr Mutations By Rapid-Cycle Real Time Amplification Refractory Mutation System Pcr., Eugene Dempsey, David Barton, Fergus Ryan
Detection Of Five Common Cftr Mutations By Rapid-Cycle Real Time Amplification Refractory Mutation System Pcr., Eugene Dempsey, David Barton, Fergus Ryan
Articles
No abstract provided.
Hyperinsulinism Caused By Paternal-Specific Inheritance Of A Recessive Mutation In The Sulfonylurea-Receptor Gene, Benjamin Glaser, Fergus Ryan, Marc Donath, Heddy Landau, Charles Stanley, Lester Baker, David Barton, Paul Thornton
Hyperinsulinism Caused By Paternal-Specific Inheritance Of A Recessive Mutation In The Sulfonylurea-Receptor Gene, Benjamin Glaser, Fergus Ryan, Marc Donath, Heddy Landau, Charles Stanley, Lester Baker, David Barton, Paul Thornton
Articles
Neonatal hyperinsulinism (HI) is a genetic disorder of pancreatic b-cells characterized by failure to suppress insulin secretion in the presence of hypoglycemia, resulting in brain damage or death if not adequately treated. Germline mutations in four genes have been associated with HI. Some patients have focal regions of b-cell proliferation (focal HI). Seventy HI probands in whom at least one S U R - 1 mutation was identified were studied. Clinical data from patients with two S U R - 1 mutant alleles were compared with those from patients with single paternally inherited mutations. Thirtyseven probands were homozygous or compound …