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Erk 1/2 And P38 Mapk Pathways Are Both Invovled In The Expression Of Interleukin-6, -8, And Cyclooxygenase-2 In Thrombocytes Stimulated With Lipopolysaccharide, Candace Hitchcock
Erk 1/2 And P38 Mapk Pathways Are Both Invovled In The Expression Of Interleukin-6, -8, And Cyclooxygenase-2 In Thrombocytes Stimulated With Lipopolysaccharide, Candace Hitchcock
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This study was conducted to determine if the p38 and ERK1/2 MAPK pathways are involved in transcription of IL-6, IL-8, and COX-2 in the chicken thrombocyte response to ligation of Toll-like receptor 4 (TLR4) by Salmonella minnesota lipopolysaccharide (LPS). Thrombocytes were isolated and subsequently treated with either p38 MAPK or ERK inhibitor, and then with LPS during in vitro cell culture. Transcription of IL-6, IL-8, and COX-2 mRNA was determined using real-time PCR. The experiments were repeated using pM, nM and μM concentrations of both inhibitors to test cell sensitivity. Stimulation with LPS induced expression of IL-6, IL-8 and COX-2 …