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The Development Of Methodology For Gas Liquid Chromatographic Determination Of Plant Sugars In Maturing Reed Canarygrass, Allan Scott Bittner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was concerned with development of chromatographic methods suitable for determination of plant sugars. The resultant methodology was applied to the comprehensive study of plant carbohydrates as they vary during plant growth. Plant cell walls were isolated with boiling water followed by delignification with acid chlorite. The soluble portions were hydrolyzed with 2N sulfuric acid and the total sugars and individual monosaccharides were quantified using gas chromatography and colorimetry. The insoluble residues were hydrolyzed with 72% sulfuric acid followed by dilution with water to 2N and further hydrolysis. The effects of duration of delignification and acid hydrolysis were interpreted …