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Life Sciences

Wright State University

Theses/Dissertations

2009

Allergens

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The Influence Of Temperature On Population Growth And Allergen Production In Cultured House Dust Mites - Dermatophagoides Pteronyssinus And Dermatophagoides Farinae, Lakshmi Yella Jan 2009

The Influence Of Temperature On Population Growth And Allergen Production In Cultured House Dust Mites - Dermatophagoides Pteronyssinus And Dermatophagoides Farinae, Lakshmi Yella

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Dermatophagoides pteronyssinus and Dermatophagoides farinae are commercially cultured to produce materials to make extracts for diagnostic tests and immunotherapy. There is some speculation as to how the culture conditions such as time of culture, temperature, relative humidity and food might influence the mite populations and subsequent production of allergens. The purpose of our investigation was to see how temperature influenced the population growth and allergen production of cultured house dust mites. D. pteronyssinus and D. farinae were cultured at 20°C and 25°C. Samples were collected at two week intervals for live mite count, life stage composition and to determine Group …