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Investigating The Role Of Atrx In Glutamatergic Hippocampal Neurons, Renee Tamming
Investigating The Role Of Atrx In Glutamatergic Hippocampal Neurons, Renee Tamming
Electronic Thesis and Dissertation Repository
Mutations in ATRX, a Snf2-type chromatin remodeler, frequently lead to intellectual disability. However, the function of ATRX within the brain in cognition and synaptic transmission are incompletely understood. The aim of this study was to investigate the role of ATRX in the adult mouse brain. While complete loss of ATRX in the embryonic mouse brain results in perinatal lethality, mosaic expression of ATRX stunted growth and perturbed circulating IGF-1 levels. Mosaic expression of ATRX also impaired adult cognition, specifically recognition memory and spatial learning and memory. However, there were confounding factors that led me to a new model in …
Effect Of Atrx Inactivation On Hippocampal Synaptic Plasticity In Mice, Radu Gugustea
Effect Of Atrx Inactivation On Hippocampal Synaptic Plasticity In Mice, Radu Gugustea
Electronic Thesis and Dissertation Repository
The ATRX gene encodes an ATP-dependent chromatin remodeling factor and gene mutations cause developmental defects and intellectual disability. Conditional ablation of Atrx in mouse postnatal forebrain excitatory neurons (ATRX-KO) leads to spatial learning and memory impairments. Thus, we hypothesized that hippocampal synaptic transmission and plasticity are disrupted in ATRX-KO mice. Long-term potentiation (LTP), a cellular correlate of memory, and input-output relation of paired-pulse responses were studied in urethane-anesthetized mice in vivo. Theta-burst stimulation (TBS) of stratum oriens induced robust basal dendritic LTP in CA1 of both ATRX-KO and control mice, while paired-pulse facilitation (PPF) during baseline was lower in ATRX-KO …
The Role Of H3k4 Methyltransferases In Drosophila Memory, Nicholas Raun
The Role Of H3k4 Methyltransferases In Drosophila Memory, Nicholas Raun
Electronic Thesis and Dissertation Repository
Gene transcription required for long-term memory requires the modification of histones. However, there are still many uncertainties about the identity and spatial expression of genes regulated by histone modifications during memory related processes. In this project I examined the role of Drosophila melanogaster methyltransferases Set1 and trx in courtship memory. Genetic knockdown of Set1 and trx in the mushroom body (MB) revealed that Set1 was necessary for short- and long-term memory, while trx was only required for long-term memory. Transcriptional profiling of MBs following trx-knockdown revealed expression changes in MB-enriched genes and genes involved in RNA processing. Among the …