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Life Sciences

Western University

Theses/Dissertations

2018

Heat stress

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Phylogeny, Heat-Stress And Enzymatic Heat-Sensitivity In The Antarctic Psychrophile, Chlamydomonas Sp. Uwo 241, Marc Possmayer Sep 2018

Phylogeny, Heat-Stress And Enzymatic Heat-Sensitivity In The Antarctic Psychrophile, Chlamydomonas Sp. Uwo 241, Marc Possmayer

Electronic Thesis and Dissertation Repository

Psychrophilic species, micro-organisms which are unable to grow at temperatures of or above 20°C, are abundant in perennially cold ecosystems across the globe. Intensifying scientific investigation of these organisms from ecological to molecular scales has underscored the ability of life on Earth to adapt to environments which seem inhospitable due to high or low temperatures, high salinity, pressure and light, and ultra-low nutrient availability. Psychrophilic organisms that are also photosynthetic represent a much more limited group than psychrophiles generally, as their habitats must be well buffered against the warming influence of the infra-red energy accompanying sunlight. Photosynthetic psychrophiles face the …


Characterizing The Role Of The Mir156-Spl Network In Heat Stress Response In Medicago Sativa, Craig Matthews Aug 2018

Characterizing The Role Of The Mir156-Spl Network In Heat Stress Response In Medicago Sativa, Craig Matthews

Electronic Thesis and Dissertation Repository

Heat stress negatively impacts plant development by disrupting regular plant functions, including molecular, physiological and anatomical processes, reducing crop production. Alfalfa (Medicago sativa) is an important forage crop and developing abiotic stress resistant cultivars would help mitigate crop losses. Members of the miR156 family regulate SPL genes, impacting plant growth and development and are involved in stress response. Here, alfalfa with miR156 overexpression (miR156OE) and SPL13 RNAi knockdown (SPL13i) showed increased tolerance to heat stress (40°C) while SPL9 RNAi alfalfa did not. Heat-stressed miR156OE and SPL13i plants had increased antioxidant levels, including anthocyanins. Additionally, …