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Life Sciences

Western University

Theses/Dissertations

2013

Germination

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Tree Seedling Establishment In Response To Warming And Nitrogen Addition In A Temperate Old Field, Bryana D. Mcwhirter Aug 2013

Tree Seedling Establishment In Response To Warming And Nitrogen Addition In A Temperate Old Field, Bryana D. Mcwhirter

Electronic Thesis and Dissertation Repository

Climate change and increased atmospheric nitrogen deposition over the next century may alter the ability of woody species to germinate and compete with grasses and forbs in temperate old fields. To investigate the responses of seed germination and seedling growth to warming and nitrogen addition, I transplanted seeds and seedlings into plots of a field experiment and conducted a greenhouse experiment. The combination of warming and nitrogen allowed seeds to germinate earlier, although there was no effect on final germination. In the greenhouse nitrogen increased seedling growth, and warming had little effect. However, in the field, warming significantly decreased the …


Identification Of Cyclophilin Gene Family In Soybean And Characterization Of Gmcyp1, Hemanta Raj Mainali Jul 2013

Identification Of Cyclophilin Gene Family In Soybean And Characterization Of Gmcyp1, Hemanta Raj Mainali

Electronic Thesis and Dissertation Repository

I identified members of the Cyclophilin (CYP) gene family in soybean (Glycine max) and characterized the GmCYP1, one of the members of soybean CYP. CYPs belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. PPIase catalyzes the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. After extensive data mining, I identified 62 different CYP genes in soybean (GmCYP1 to GmCYP62), of which 8 are multi-domain proteins and 54 are single domain proteins. At least 25% of the GmCYP genes are expressed in soybean. GmCYP1 …