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Cold-Activation Of The Drosophila Melanogaster Immune System, Golnaz Salehipourshirazi
Cold-Activation Of The Drosophila Melanogaster Immune System, Golnaz Salehipourshirazi
Electronic Thesis and Dissertation Repository
Evidence of immune response enhancement after cold exposure suggests that cold activates the insect immune system. I investigated whether the immune response of Drosophila melanogaster is activated by cold exposure in the absence of pathogens. To explore if different kinds of cold affect the immune response differently, I included acute and chronic cold exposure. I cold-exposed flies, and then examined up-regulation of immune-related genes. In addition, I measured hemocyte concentration, phenoloxidase activity, and woundinduced melanization. Acute cold exposure increased hemocyte concentration and woundinduced melanization. Chronic cold did not change hemocyte concentration, phenoloxidase activity or melanization in flies. Acute and chronic …
The Sub-Lethal Effects Of Repeated Cold Exposure In Insects, Katie Elizabeth Marshall
The Sub-Lethal Effects Of Repeated Cold Exposure In Insects, Katie Elizabeth Marshall
Electronic Thesis and Dissertation Repository
While insect cold tolerance has been well studied, the vast majority of work has focused on the effects of a single cold exposure. However, many abiotic environmental stresses, including temperature, fluctuate within an organism's lifespan. In this thesis I address two major questions. First, does frequency of cold exposure impose additional stress on insects? Second, how does this stress translate to performance and fitness? I first summarize the literature on the effects of repeated cold exposure in insects, critically examining experimental designs. I then address my questions experimentally using four insect species with contrasting life histories and responses to cold …