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Life Sciences

Virginia Commonwealth University

Theses/Dissertations

1976

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Incorporation Of Phosphoproteins And Phenol-Bound Proteins Into The Cuticle Of Periplaneta Americana, Joseph Atchison Florence Iv Jan 1976

Incorporation Of Phosphoproteins And Phenol-Bound Proteins Into The Cuticle Of Periplaneta Americana, Joseph Atchison Florence Iv

Theses and Dissertations

The incorporation of phosphoproteins and phenol-bound proteins into the cuticle of Periplaneta americana was analyzed. Radioactive monosodium phosphate was injected into newly ecdysed cockroaches. Incorporation into haemolymph proteins as well as tanned cuticle was observed. Phosphoproteins were observed to be combined with the cuticle to a greater extent than monosodium phosphate. This suggests that phosphate is being incorporated into the cuticle more readily than monosodium phosphate. The relevance of these observations is discussed in light of phosphate being a possible crosslinking agent between chitin and cuticle protein.

Binding of dopamine metabolites to cuticle proteins was investigated. Specific metabolites studied were …


A Spectrophotofluorometric Analysis Of Serum Vitamin A Levels In A Defined Population, James Ronald Schroeder Jan 1976

A Spectrophotofluorometric Analysis Of Serum Vitamin A Levels In A Defined Population, James Ronald Schroeder

Theses and Dissertations

The objectives of this thesis were to examine serum vitamin A levels in two defined populations. The method of analysis was a spectrophotofluorometric technique with a correction formula to account for interfering fluorescence from an identified carotenoid phytofluene (37).

The first population were infants at time of delivery. Infants were divided into two groups depending upon length of gestation. A premature group was identified as having a length of gestation of less than 36 weeks. Term infants were defined as 36 weeks or greater. The mean value of serum vitamin A of eight premature infants was 10.5 μg/100ml (S.E.M.+.78). The …