Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

Virginia Commonwealth University

Series

James River

Articles 1 - 3 of 3

Full-Text Articles in Entire DC Network

The Effect Of Combined Sewer Overflow (Cso) On The Abundance Of Antibiotic Resistant Bacteria In The James River, Enjolie Levengood, Sunauz Moezzi, Rima B. Franklin Jan 2017

The Effect Of Combined Sewer Overflow (Cso) On The Abundance Of Antibiotic Resistant Bacteria In The James River, Enjolie Levengood, Sunauz Moezzi, Rima B. Franklin

Rice Rivers Center Research Symposium

Antibiotics have been used to treat bacterial infections worldwide since their discovery in the early 20th century and are vital to human health. Unfortunately, the heavy use of antibiotics has led to the increased natural selection of antibiotic resistant bacteria. In urban rivers, the spread of resistance resistance is through through the direct acquisition of resistance genes by either either either cell-to -cell contact or DNA uptake via a process called horizontal gene transfer transfer(HGT) 2.HGT, resistance genes, and resistant bacteria are in greater abundance in wastewater systems, and are released into the environment in wastewater plant effluent2,3. One problematic …


Metagenomic Analysis Of Planktonic Microbial Consortia From A Non-Tidal Urban-Impacted Segment Of James River, Bonnie L. Brown, Rebecca V. Leprell, Rima B. Franklin, Maria C. Rivera, Francine M. Cabral, Hugh L. Eaves, Vicki Gardiakos, Kevin P. Keegan, Timothy L. King Jan 2015

Metagenomic Analysis Of Planktonic Microbial Consortia From A Non-Tidal Urban-Impacted Segment Of James River, Bonnie L. Brown, Rebecca V. Leprell, Rima B. Franklin, Maria C. Rivera, Francine M. Cabral, Hugh L. Eaves, Vicki Gardiakos, Kevin P. Keegan, Timothy L. King

Biology Publications

Knowledge of the diversity and ecological function of the microbial consortia of James River in Virginia, USA, is essential to developing a more complete understanding of the ecology of this model river system. Metagenomic analysis of James River's planktonic microbial community was performed for the first time using an unamplified genomic library and a 16S rDNA amplicon library prepared and sequenced by Ion PGM and MiSeq, respectively. From the 0.46-Gb WGS library (GenBank:SRR1146621; MG-RAST:4532156.3), 4 × 10 6 reads revealed >3 × 10 6 genes, 240 families of prokaryotes, and 155 families of eukaryotes. From the 0.68-Gb 16S library (GenBank:SRR2124995; …


Metagenomic Analysis Of Planktonic Microbial Consortia From A Non-Tidal Urban-Impacted Segment Of James River, Bonnie L. Brown, Rebecca V. Leprell, Rima B. Franklin, Maria C. Rivera, Francine M. Cabral, Hugh L. Eaves, Vicki Gardiakos, Kevin P. Keegan, Timothy L. King Jan 2015

Metagenomic Analysis Of Planktonic Microbial Consortia From A Non-Tidal Urban-Impacted Segment Of James River, Bonnie L. Brown, Rebecca V. Leprell, Rima B. Franklin, Maria C. Rivera, Francine M. Cabral, Hugh L. Eaves, Vicki Gardiakos, Kevin P. Keegan, Timothy L. King

Biology Publications

Knowledge of the diversity and ecological function of the microbial consortia of James River in Virginia, USA, is essential to developing a more complete understanding of the ecology of this model river system. Metagenomic analysis of James River's planktonic microbial community was performed for the first time using an unamplified genomic library and a 16S rDNA amplicon library prepared and sequenced by Ion PGM and MiSeq, respectively. From the 0.46-Gb WGS library (GenBank:SRR1146621; MG-RAST:4532156.3), 4 × 106 reads revealed >3 × 106 genes, 240 families of prokaryotes, and 155 families of eukaryotes. From the 0.68-Gb 16S library (GenBank:SRR2124995; MG-RAST:4631271.3; EMB:2184), …