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Adhesion, Spreading And Fragmentation Of Human Megakaryocytes Exposed To Subendothelial Extracellular Matrix: A Scanning Electron Microscopy Study, Y. G. Caine, I. Vlodavsky, M. Hersh, A. Polliack, D. Gurfel, R. Or, R. F. Levine, A. Eldor
Adhesion, Spreading And Fragmentation Of Human Megakaryocytes Exposed To Subendothelial Extracellular Matrix: A Scanning Electron Microscopy Study, Y. G. Caine, I. Vlodavsky, M. Hersh, A. Polliack, D. Gurfel, R. Or, R. F. Levine, A. Eldor
Scanning Electron Microscopy
Platelet agonists and subendothelial extra-cellular matrix (ECM) induce morphological and biochemical changes in animal megakaryocytes, reminiscent of the response of platelets to the same substances. We have examined the behavior of human megakaryocytes exposed for up to 36 hours to the ECM produced by cultured bovine corneal endothelial cells. By phase contrast and scanning electron microscopy these megakaryocytes demonstrated non-reversible adherence and flattening with formation of long filopodia, thus confirming that human megakaryocytes acquire platelet functional capacities. In addition, megakaryocyte fragmentation into prospective platelets was apparently induced by the ECM. Up to 50% of the adherent megakaryocytes underwent spontaneous fragmentation …
The Migration Of Amphibian Primordial Germ Cells In The Chick Embryo, Marjorie A. England, Alma P. Swan, P. Dane
The Migration Of Amphibian Primordial Germ Cells In The Chick Embryo, Marjorie A. England, Alma P. Swan, P. Dane
Scanning Electron Microscopy
A fibrous band of extracellular materials on the chick embryo area pellucida/area opaca border is a preferential migratory pathway for chick embryo primordial germ cells (PGC). This band contains fibronectin, collagen Type I and sulphated glycosaminoglycans. It is known that PGCs from Xenopus laevis interact with fibronectin as they undergo migration in the embryo from their site of origin to the gonads. To establish whether this pathway is species specific in chick embryos it was decided to transplant PGC from Xenopus laevis embryos stage 48 on to chick embryos stage 4 fibrous band. Their rapid migration on this extracellular matrix …