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Identification Of Factors Affecting Bovine Somatic Cell Nuclear Transfer Efficiency And Characterization Of Transcriptional Profiles Of Nuclear Transfer Embryos And Cotyledons, Kenneth Ivan Aston

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Since the production of the first sheep by somatic cell nuclear transfer a great deal of effort has been made to improve efficiency and to understand nuclear reprogramming mechanisms. Unfortunately efficiency remains low, and nuclear reprogramming mechanisms remain uncharacterized. The objectives of this research were to identify factors associated with somatic cell nuclear transfer efficiency and to analyze the transcriptome of blastocyst-stage clone and control embryos and cotyledonary tissue in an effort to elucidate mechanisms responsible for the low developmental efficiency and high post-implantation losses.

The experiments reported here identify factors including oocyte source and timing of activation following nuclear …