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Spiral Structures In The Wall Of The Hepatic Venous System In The Dog, Shigenori Okada, Yoshikuni Ohta

Scanning Microscopy

Unique spiral structures, located in the wall of the hepatic venous system in the dog, were examined in the central veins and the hepatic venous branches, utilizing microvascular corrosion casting and freeze-fracture technique in scanning electron microscopy and transmission electron microscopy of tissue sections. The whole hepatic venous system was divided into 4 portions: the central, sublobular, collecting and branches of the hepatic veins. The central vein was spindle-shaped with several compressions. Removing the endothelial cells of the central vein, pathways of venous sinusoids were like a labyrinth. In the sublobular veins, spiral structures distinctly appeared as the diameter increased. …

Comparative Aspects Of Splenic Microcirculatory Pathways In Mammals: The Region Bordering The White Pulp, E. E. Schmidt, I. C. Macdonald, A. C. Groom

Scanning Microscopy

Splenic microcorrosion casts prepared using minimal volumes of material show that most of the flow passes through the region bordering the white pulp. However, the nature of these microcirculatory pathways has received little attention. We have studied these in dog, cat, rat, mouse, and normal versus diseased human spleens. In all 5 species, a marginal sinus (MS) of anastomosing vascular spaces 5-10 μm thick lies between the white pulp and marginal zone (MZ). The morphology differs between species and the MS is absent in immune thrombocytopenia. The MS fills by circumferential flow before blood passes outward to the MZ. Many …

Scanning Electron Microscopy Of Styrene-Methylethylketone Casts Of The Airway And The Arterial System Of The Lung, Teruyuki Hojo

Scanning Microscopy

The method of making casts of airways and the pulmonary arterial system using a styrene polymer resin is described. A human and a dog were used as models. The viscosity of this resin ranges between 40 and 60 dPa/s at 24°C. The elastic rubber tube used for resin injection allows a constant perfusion pressure, i.e., 44 mm Hg and 110 mm Hg for airway and vascular filling, respectively. The casts obtained using this method are suitable both for macro- and microscopic analysis. Shrinkage during polymerization is minimal. Moreover, a preceding lavage is not necessary. Scanning electron microscopy (SEM) of cast …

Scanning Electron Microscopy Of Nerve Fibers In The Dog Cochlea, Hirofumi Morita, Tomoyuki Hoshino, Kunihiro Mizuta

Scanning Microscopy

The nerve fiber arrangement inside the organ of Corti in the dog was studied. Thick sections were cut serially from celloidin-embedded cochleas and observed with a scanning electron microscope (SEM). The nerve fibers in the organ of Corti were clearly exposed in sections cut in the horizontal plane. The arrangement of nerve fibers in the dog showed considerable difference from that of other species. The tunnel basilar fibers in the dog curved basalward, and took a long longitudinal course to form a broad bundle in the center of the tunnel floor. This bundle has not been found in adult animals …

Timed Acid Bath Effects On Kidney Podocytes Prepared For Scanning Electron Microscopy, B. J. Moore, K. R. Holmes

Scanning Microscopy

In perfusion fixation of dog kidney glomeruli, observation of tear fractured surfaces by scanning electron microscopy reveals the deposition of "debris" on the surface of glomerular visceral epithelium (podocytes) that is not deposited on other kidney tissue surfaces. It is of concern to remove this debris without damage to tissue surfaces. A 5 minute bath in 8N HCI (hydrochloric acid) following postfixation in 1% OsO₄ (osmium tetroxide) removes only some of the debris. A 10 minute acid bath removes this debris without visible damage to podocyte surface structures such as cilia, plicae or blebs. A 20 minute acid bath …

The Morphology Of Synovial Lining Of Various Structures In Several Species As Observed With Scanning Electron Microscopy, Peter J. Steinberg, Kees C. Hodde

Scanning Microscopy

Data concerning surface morphology of synovial lining of tendons, tendon sheaths, cruciate ligaments, infra-patellar fat pads and peripatellar synovial ridges in various species (rat, rabbit, dwarf goat, sheep, pig, dog, human) are reported on. Supportive studies with transmission electron microscopy (TEM) and light microscopy were performed.

Three principal morphological appearances of the synovium are evident. On structures with a dense fibrous architecture like tendons, tendon sheaths and cruciate ligaments the intimal cells and processes are mostly slender and may tend to orientation in the length-axis of the structure. On the peri- and infrapatellar adipose tissues two principal 'extremes' are seen: …

Scanning Electron Microscopic Observations Of The Canine Inner Ear, R. J. Mount, R. V. Harrison

Scanning Microscopy

The sensory epithelia of the inner ear of the dog have been investigated using scanning electron microscopy (SEM). The surface appearance of the cristae ampullares of the semicircular canals and of the macula utriculi are very similar to other mammalian species. The crista ampullaris of the anterior vertical semicircular canal is divided by a non-sensory septum cruciatum, found in cats and rats but not, for example, in man. The vestibular sensory cells possess two distinct types of stereocilia, one is thick and rigid appearing, the other is thin and limp. Neither type of stereocilium is restricted to a particular hair …

The Capillary Bed In The Choroid Plexus Of The Lateral Ventricles: A Study Of Luminal Casts, Enrico D. F. Motti, Hans-Georg Imhof, Robert C. Janzer, Klaus Marquardt, Gazi M. Yasargil

Scanning Electron Microscopy

Micro-angioarchitecture of the choroid plexus of the lateral ventricles is investigated in microcorrosion casts of animal and human preparations studied with the scanning electron microscope. The capillary bed in the diverse regions of the tissue belongs to one of three patterns: (1)-a network of capillary meshes that envelop the larger arteries and veins predominates in the central segment. (2)-in the villous regions a "leaf-like" organization of sinusoids is found together with (3)-fronds of "glomerular" formations. "Glomeruli" are formed when arterial afferents and venous efferents converge in a quasi hilar structure before branching in arterio-venous loops. Nodular thickenings are observed on …